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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 176-181.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

发育对番茄叶片防御相关酶活性的影响

赵文杰 陈靠山 张鹏英   

  • 收稿日期:2010-12-14 修回日期:2011-02-17 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    促生、抗逆、防治病虫害的多功能生化-生物制剂的研制;利用基因芯片检测牛蒡低聚果糖诱导烟草抗病性相关基因

Developmental-depend Changes of Defense-related Enzymes in Tomato Leaf

  • Received:2010-12-14 Revised:2011-02-17 Online:2011-07-05 Published:2011-07-05

PDF (PC)

1078

摘要：

以不同发育阶段及开花期番茄植株为材料，研究了不同发育时期番茄防御酶同工酶酶谱及防御酶活性的差异。结果发现，同叶龄番茄叶片过氧化物酶、超氧化物歧化酶和酯酶同工酶酶活性随植株生长均显著增加；叶龄较植株发育阶段对三种同工酶酶谱的影响较小。同叶龄番茄叶片的β-1,3-葡聚糖酶和几丁质酶在不同发育阶段有显著差异，β-1,3-葡聚糖酶和几丁质酶活性分别在开花初期和六叶期植株中最高；β-1,3-葡聚糖酶活性随着叶龄的增加而升高，而几丁质酶活性变化不大。该研究表明，植物的一些抗病防御相关因子与植物发育时期密切相关，植株发育阶段对叶片防御相关因子的影响显著高于叶龄对它的影响。

关键词: 筛选, 筛选

Abstract:

Several resistance related factors including isozyme of Peroxidase (POD), Superoxide dismutase (SOD), and Esterase (EST), β-1,3-glucanase and chitinase activities in leaf of tomatoes at different development stages were analyzed. The results demonstrated that the isozymes of POD, SOD and EST in the same age leaves were enhanced with plant growth. Effects of leaf age on these isozymes were much lower than plant developmental stage. The activities of β-1,3-glucanase were enhanced significantly in anthesis plant, while chitinase were the highest of all in six-leaf plant. β-1,3-glucanase activities were enhanced with leaf age increasing. However, chitinase activities were not significantly different in three age leaf.

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