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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (20): 109-114.

• 食品 营养 检测 安全 • 上一篇    下一篇

SPE-GC-MS法和HS-SPME-GC-MS法测定辐照葵花籽中挥发性碳氢化合物

李庆鹏 哈益明 范蓓 李淑荣 周洪杰 李安 王锋 朱捷   

  • 收稿日期:2011-01-12 修回日期:2011-02-21 出版日期:2011-08-15 发布日期:2011-08-15
  • 基金资助:

    中国农业科学院基本科研业务费专项资助（

Detection of Volatile Hydrocarbons in Irradiated Sunflower Seeds by SPE-GC-MS and HS-SPME-GC-MS Methods

  • Received:2011-01-12 Revised:2011-02-21 Online:2011-08-15 Published:2011-08-15

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摘要：

挥发性碳氢化合物是含脂食品辐照鉴定的标识性化合物，采用两种不同的前处理方法即SPE（固相萃取）和SPME（固相微萃取），对葵花籽辐照前后的6种碳氢化合物即1-C14:1（1-十四烯）、C15:0（正十五烷）、1-C16:1（1-十六烯）、C17:0（正十七烷）、1,7-C16:2（1,7-十六二烯）和8-C17:1（8-十七烯）进行了GC-MS（气相色谱质谱）分析，建立了能判别葵花籽是否经过辐照的鉴定方法，同时还研究了不同辐照剂量和炒制加工处理对葵花籽中6种碳氢化合物的影响。研究结果表明，SPE-GC-MS法和HS-SPME-GC-MS法均能用于葵花籽的辐照鉴定，其中HS-SPME-GC-MS法操作简便，能对葵花籽中的碳氢化合物进行准确快速的定性分析。未辐照葵花籽中检出了4种碳氢化合物，分别是1-C14:1、C15:0、1-C16:1、C17:0，而1,7-C16:2和8-C17:1仅在葵花籽辐照后产生，是进行辐照鉴定的最佳标识物。剂量对碳氢化合物的变化影响明显，随着辐照剂量的增加，1,7-C16:2和8-C17:1的水平显著提高，为辐照葵花籽的定量检测提供了理论依据。

关键词: 产量, 产量

Abstract:

Two pretreatment methods which were SPE (solid-phase extraction) and SPME (solid-phase micro-extraction) had been studied to identify irradiated sunflower seeds by GC-MS (gas chromatography mass spectrum) analysis of six hydrocarbons, which were 1-C14:1 (1-tetradecene), C15:0 (n-pentadecane), 1-C16:1 (1-hexadecene), C17:0 (n-heptadecane), 1,7-C16:2 (1,7-hexadecadiene) and 8-C17:1 (8-heptadecene) between unirradiated and irradiated samples. Meanwhile, the effect of irradiation dose and frying process on hydrocarbons in sunflower seeds were also studied. Results showed that these two methods could both be used to identify whether sunflower seeds were irradiated or not, and HS-SPME-GC-MS method was easy to operate for quick and extract qualitative analysis. Irradiation dose had significant affected on hydrocarbons. Four hydrocarbons (1-C14:1, C15:0, 1-C16:1 and C17:0) were detected in unirradiated sunflower seeds. 1,7-C16:2 and 8-C17:1 were only detected in irradiated samples. Therefore, the two hydrocarbons were the best markers for detection of irradiated sunflower seeds.

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