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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (29): 42-45.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

大鼠GTH细胞内PKC的改变对LH表达的影响

王新 刘金玲 韦旭斌 谭建华 赖小平   

  • 收稿日期:2011-04-15 修回日期:2011-05-03 出版日期:2011-11-15 发布日期:2011-11-15
  • 基金资助:

    国家自然科学基金“不同脉冲频率GnRH调节大鼠GTH表达的信号转导机制研究”

  • Received:2011-04-15 Revised:2011-05-03 Online:2011-11-15 Published:2011-11-15

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969

摘要：

目的是分析大鼠分泌LH的GnRH受体后信号转导机制。将GTH细胞内PKC兴奋或抑制后，用高频GnRH脉冲刺激，再用ELISA法检测其LH分泌量，并与空白组比较。结果表明，细胞PKC活性显著影响LH的分泌。得出结论，PKC-Ca2+是GnRH脉冲刺激引起的LH分泌的GnRH受体后信号转导途径之一。

关键词: 白肋烟, 白肋烟, 转化株, 干旱, 降烟碱, 烟碱转化率, 稳定性

Abstract:

The aim: To analyze the GnRH post-receptor signal transduction mechanism of the secretion of LH in the mammalian. The way: The GTH cells was stimulated by GnRH at high frequencies after PKC in them was excited or inhibited. LH was detected by ELISA, which was compared with the blank comparison group. Result: The PKC level in GTH cells influences the expression of LH significantly. Conclusion: PKC-Ca2+ is one of the GnRH post-receptor signal transduction pathways that the cells stimulated by GnRH impulse secret LH.