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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (24): 273-276.

• 植物保护 农药 • 上一篇    下一篇

烟蚜两种微卫星标记银染方法的比较

吕召云 杨茂发 商胜华   

  • 收稿日期:2011-04-20 修回日期:2011-05-08 出版日期:2011-09-25 发布日期:2011-09-25
  • 基金资助:

    中国烟草总公司贵州省公司科技项目

Comparison of Two Silver Staining Methods for Polyaerylamide Gels Electrophoresis by Using SSR markers on Myzus persicae (Sulzer)

  • Received:2011-04-20 Revised:2011-05-08 Online:2011-09-25 Published:2011-09-25

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摘要：

实验比较了烟蚜SSR-PCR产物聚丙烯酰胺凝胶电泳两种改良银染方法，目的是为初用者在选择银染方法时提供参考。用非变性聚丙烯酰胺凝胶电泳检测烟蚜SSR-PCR的产物，结果表明银染方法二灵敏度高、简便省时，更适于对烟蚜遗传多样性的研究。

关键词: 内生菌, 内生菌, 蕨类植物, 多样性, 活性成分

Abstract:

Two ways of DNA improved silver staining methods for polyacrylamide gels electrophoresis on Myzus persicae (Sulzer) were compared in this article for first detection. The detected results with the technique of SSR markers by non-denaturant polyacrylmaaide gel eletrophoresis indicated that the second method was characterized as more sensitive and simple steps when compared with the first method. And for saving time and work the second method was more appropriate to the genetic diversity of Myzus persicae (Sulzer).