关键词: 小麦, 小麦, 航天诱变, 醇溶蛋白, SRAP

Abstract:

To find a new simple, fast and high quality genomic DNA extraction technique from dried seeds, this experiment was carried out. The seeds were ground to powder. 100 mg powder of seeds was put in an eppendorf tube and the genomic DNA was extracted by our method, respectively. The final eluate was detected by ultramicro UV/Vis spectrophotometer. The purity and quality of the genomic DNA extracted was confirmed by PCR and enzyme digestion analysis. 619.67 to 1811.21 ng of genomic DNA was extracted from 100 mg dried seeds for all individuals sampled, and the A260/A280 ratio of DNA samples was from 1.87 to 2.07. The purity and quality of the genomic DNA solution could be considered so high that it was sufficient for enzyme digestion and PCR analysis. The target bands all could be amplified from plant seeds by using their specific endogenesis gene primers. And the total DNA could be digested thoroughly by endonuclease such as EcoRV and HindIII. The results showed that genomic DNA was extracted successfully from dried seeds by this approach.