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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (4): 200-205.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

中国局部地区草坪立枯丝核菌的rDNA ITS序列分析

淮稳霞 程衬衬 李东林 赵文霞   

  • 收稿日期:2011-05-18 修回日期:2011-11-29 出版日期:2012-02-05 发布日期:2012-02-05

Sequences Analysis of rDNA Internal Transcribed Spacer (ITS) of Rhizoctonia solani Kühn from Some Local Areas in China

  • Received:2011-05-18 Revised:2011-11-29 Online:2012-02-05 Published:2012-02-05

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摘要：

为了鉴定草坪褐斑立枯丝核病菌的融合群类型并探讨其系统发育关系，本研究对11株分离自草坪草和4株分离自土壤的立枯丝核菌（Rhizoctonia solani）进行了rDNA-ITS测序，结合GenBank中登录的立枯丝核菌14融合群或亚群的代表性菌株及禾谷丝核菌（R. cerealis）、玉蜀黍丝核菌（R. zeae）和水稻丝核菌（R. oryzae）3个近缘种，共45条ITS序列进行了分析，利用MEGA 4.1软件建立ITS聚类分析树状图。结果表明，立枯丝核菌各融合群代表菌株及所有供试菌株聚为一组；该组又可细分为14个遗传聚亚类，隶属同一融合群的代表菌株聚在同一亚组，供试15株立枯丝核菌归入AG2-2和AG1-IA两个融合群。这些结果为该病害的科学防控提供了可靠依据，并进一步验证了ITS序列分析法是区分立枯丝核菌不同融合群或亚融合群菌株的有力工具。

关键词: 巴西蕉, 巴西蕉, 短期高温, 光合作用, 生理指标

Abstract:

The internal transcribed spacer (ITS) region of the ribosomal DNA from 11 isolates of Rhizoctonia solani Kühn from turfgrass and 4 from soil were sequenced in order to identify anastomosis groups and discuss phylogenetic relationships between them. Based on the 15 sequences and 30 published sequences of R. solani from 14 anastomosis groups or sub-groups, R. cerealis, R. zeae and R. oryzae in GenBank database, the ITS phylogenetic tree were generated with MEGA 4.1. The result showed that, all the representative isolates of known anastomosis groups and 15 samples tested were clustered into one group. In this group, representatives of the different anastomosis groups clustered separately, and 15 isolates sequenced were divided into AG2-2 and AG1-IA. These results provided a reliable basis for disease control, and also suggested that the sequence analysis of ITS rDNA region was a valuable tool for identifying anastomosis groups of R. solani.