关键词: 几丁质酶, 几丁质酶, 几丁质酶产生菌, 发酵条件, 优化

Abstract:

Establishment of fingerprinting is accurate and reliable basis for cultivar assessment and new plant variety protection. Thirty-five elite maize inbred lines and 19 hybrids were analyzed with 74 pairs of primers respectively by SSR markers to establish the standard DNA fingerprinting for seed test. Ten and 20 pairs of primers distributed on ten chromosomes in maize genome were finally screened to develop numbered fingerprinting of inbred lines and hybrids. There were 115 and 124 alleles in inbred lines and hybrids fingerprinting respectively. The probability for the fingerprinting of each line and hybrid was 3.13×10-11 and 5.59×10-25, which was feasible to be used in variety identification. The established fingerprinting had a tremendous potential in new variety assessment and intellectual property rights protection.