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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 254-257.

所属专题： 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

香蕉枯萎病诱导抗性研究

王芳 李静 梁妹燕   

  • 收稿日期:2011-05-18 修回日期:2011-07-22 出版日期:2011-11-05 发布日期:2011-11-05
  • 基金资助:

    佛山市科技发展专项基金

Studies on Induced Resistance of Fusarium oxysporum f. sp. cubense

  • Received:2011-05-18 Revised:2011-07-22 Online:2011-11-05 Published:2011-11-05

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摘要：

以防御酶为指标，研究香蕉枯萎病的诱导抗性，以期开发香蕉枯萎病的生防制剂。分别接种串珠镰刀菌、饱和石灰水、无菌水处理组培香蕉苗，7天后接种尖孢镰刀菌，测定过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性。结果表明：串珠镰刀菌和饱和石灰水对香蕉枯萎病株POD酶有一定影响，但在3~6 min均未达到5%的显著水平；25℃反应15 min，串珠镰刀菌处理SOD的活力（22.967）高于石灰水（18.171）和无菌水（14.322）；2种处理CAT酶的活力曲线略高于对照，但处理间的差异不明显。说明串珠镰刀菌、饱和石灰水能在一定程度上诱导抗性的产生。研究旨在为香蕉枯萎病诱导抗性提供一定的理论依据。

关键词: 序列分析, 序列分析

Abstract:

The study was done for the induced resistance of Fusarium oxysporum f. sp. cubense for defense enzymes as an indicator, in order to develop biocontrol agents. Inoculated Fusarium moniliforme, saturated limewater and sterile water respectively to banana seedlings. After 7 days, inoculated Fusarium oxysporum, determinated peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) activity. The results showed Fusarium moniliforme and saturated limewater had a certainly affection on POD enzymes, but in 3-6 min, all did not reach significant level of five percent, in 25℃ reached 15 min, the SOD activity of Fusarium moniliforme (22.967) was higher than saturated limewater (18.171) and sterile water (14.322) .CAT activity curve of two treatments were slight higher than comparison, but between treatments had not significant difference, showed limewater and Fusarium moniliforme can produce induced resistance certainly. The purpose of the research laid the foundation for the induced resistance of Fusarium oxysporum f. sp. cubense.