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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 303-307.

• 植物保护 农药 • 上一篇    下一篇

赫氏埃希菌抗体的体外特异性抑菌试验

陈煜 张彦明 肖西志 高宏伟 邓明俊 吴振兴   

  • 收稿日期:2011-08-22 修回日期:2011-09-13 出版日期:2011-10-25 发布日期:2011-10-25
  • 基金资助:

    中国检验检疫科学研究院基础研究发展项目;福建农林大学青年教师基金

Studies on specific antimicrobial effect in vitro caused by antibody of E.hermannii

  • Received:2011-08-22 Revised:2011-09-13 Online:2011-10-25 Published:2011-10-25

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722

摘要：

为研究赫氏埃希菌抗体在体外对赫氏埃希菌的抑制作用，制备了兔抗赫氏埃希菌多克隆抗体，并用Protein G树脂抗体纯化柱进行纯化，纯化后的抗体与赫氏埃希菌混合，在不同的时间点对混合物进行扫描电镜分析和培养基生长分析。同时，用赫氏埃希菌伤口感染20只Balb/c小白鼠做赫氏埃希菌多克隆抗体的治疗效果分析。结果表明，赫氏埃希菌抗体在加入后的20 min开始裂解细菌，并在80 min后全部将赫氏埃希菌裂解。混合物的细菌生长分析表明没有有活性的赫氏埃希菌存在。小鼠感染实验表明被赫氏埃希菌感染的小鼠在应用该抗体治疗后，恢复时间明显比未用抗体治疗的对照组快。因此，所制备的赫氏埃希菌多克隆抗体将能作为一种免疫治疗的方法应用于临床治疗。

关键词: 虾青素, 虾青素, 纯化, 皂化, 红酵母NZ-01

Abstract:

To study the specific antimicrobial effect in vitro caused by antibody of E.hemannii. The polyclonal rabbit antibody against E.hermannii was prepared and puried with Protein Gresin subsequently. Scanning electron microscope and nutrient agar media were used to evaluate the mixture of puried antibody and E.hermannii at different time points. Twenty Balb/c rats infected by E.hermannii through wound on their backs were used to evaluate the therapy effect of the prepared antibody of E.hermannii. The result showed that E.hermannii began to lyse at 20 minutes and was disintegrated completely at 80 minutes. In addition, there was no alive E.hermannii growed on nutrient agars where the mixture was plated. Infection assay against rats showed that the recovery time of infected rats which were treated by prepared antibody was shorter than those of without antibody introduced. Prepared polyclonal antibody against E.hermannii could be used as one of immune therapy methods for clinical cases caused by E.hermannii.