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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (9): 214-217.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

茉莉酸甲酯对人参β-1,3-葡聚糖酶和几丁质酶活性的影响

傅俊范 孙嘉曼 周如军 严雪瑞   

  • 收稿日期:2011-08-29 修回日期:2011-11-16 出版日期:2012-03-25 发布日期:2012-03-25
  • 基金资助:

    辽宁省科技攻关项目

Effect of Methyl Jasmonate on the Activities of β-1, 3-glucanase and Chitinase in Ginseng

  • Received:2011-08-29 Revised:2011-11-16 Online:2012-03-25 Published:2012-03-25

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摘要：

为了明确β-1,3-葡聚糖酶和几丁质酶在茉莉酸甲酯（MeJA）诱导人参抗人参锈腐病过程中的作用，试验设4个处理：MeJA、Cylindrocarpon destructans、MeJA+Cylindrocarpon destructans和CK。MeJA浓度为200 mg/L。处理后3，6，9，12，15，20，25，30天测定酶活。结果表明，经MeJA处理后接种人参锈腐菌，人参根系β-1,3-葡聚糖酶和几丁质酶的活性均较对照增强。β-1,3-葡聚糖酶活性在第12天达到峰值，是对照的1.39倍；几丁质酶活性在第15天达到峰值，是对照的1.51倍。这表明β-1,3-葡聚糖酶和几丁质酶在MeJA诱导人参抗人参锈腐病的过程中起重要作用。

关键词: 抗氧化, 抗氧化

Abstract:

The aim was to study the function of β-1,3-glucanase and chitinase in ginseng resistance to Cylindrocarpon destructans induced by Methyl Jasmonate. Four treatments were designed in the test, including MeJA、Cylindrocarpon destructans、MeJA+ Cylindrocarpon destructans and CK。Ginseng plants were immersed in Methyl Jasmonates solution (200 mg/L). The ginseng plants were obtained after 3, 6, 9, 12, 15, 20, 25 and 30 days. The activities of β-1,3-glucanase and chitinase were measured. The results showed that both the activities of β-1, 3-glucanase and chitinase could be increased significantly in the ginseng roots induced by MeJA than those in control. The activities of β-1, 3-glucanase reached the peak at 12 days after inoculation, of which was 1.39 times of CK; and chitinase activity reached the peak at 15 days after inoculation, was 1.51 times of CK. The findings indicated that β-1,3-glucanase and chitinase play critical roles in resistance to C. destructans in ginseng induced by Methyl Jasmonate.