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中国农学通报 ›› 2013, Vol. 29 ›› Issue (36): 295-301.doi: 10.11924/j.issn.1000-6850.2013-0861

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

植物细胞质雄性不育育性恢复基因研究进展

陈智华 赵丽梅 许志茹 张春宝   

  • 收稿日期:2013-03-26 修回日期:2013-04-20 出版日期:2013-12-25 发布日期:2013-12-25
  • 基金资助:
    国家自然科学基金;国家高技术研究发展计划重点项目;中国博士后基金;吉林省青年基金

Advance in the Fertility Restorer Gene of Cytoplasmic Male Sterility in Plant

  • Received:2013-03-26 Revised:2013-04-20 Online:2013-12-25 Published:2013-12-25

摘要: 杂种优势已广泛应用于农业生产,这其中主要依靠细胞质雄性不育系统。细胞质雄性不育“三系”是杂交种选育的基础材料。其中,强恢复系的选育非常繁琐,且恢复力只能通过与不育系的测交来鉴定,既耗时又费力,因此,人们对育性恢复基因进行了大量的研究。本文归纳了恢复基因的结构特征、作用机理、遗传模式、基因定位及克隆上的研究进展和存在问题。认为随着二代测序技术的发展,可以利用全基因组重测序和转录组测序等技术开发新型分子标记进行恢复基因精细定位,或直接通过测序技术鉴定恢复基因。这将为恢复系的分子标记辅助选育和利用基因工程手段人工改良和创制恢复系提供帮助,也将为研究细胞质雄性不育育性恢复基因的遗传、进化和特征,全面解析植物细胞质雄性不育育性恢复机理奠定基础。

关键词: 化学成分, 化学成分

Abstract: Heterosis has been widely used in agricultural production, which relies mainly on the cytoplasmic male sterility System. Cytoplasmic male sterility "three-lines” are the basic materials for hybrid breeding. Breeding for the strong restorer line is very complicated and restoring ability can only be identified by testcross with the male sterile line, which is time-consuming and labor-intensive. Therefore, researchers do a lot of studies on the fertility restorer gene. This paper summarizes the restorer gene structural features, mechanism of action, mode of inheritance, gene mapping and cloning research and concluded that with the development of second-generation sequencing technology, it can be used the whole genome re-sequencing and RNA-seq technologies to develop new molecular markers for fine mapping of genes, or directly cloning restorer genes. It will play an important role to marker-assisted breeding and production of new restorer lines by genetic engineering. It also will lay a foundation for exploring the fertility restoration mechanism by studying the inheritance, evolution and characteristics of restorer gene.

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