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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 280-285.doi: 10.11924/j.issn.1000-6850.2014-1001

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抗FOC4内生放线菌NJQG-3A1的定殖与防效研究

胡一凤,井 涛,周登博,张锡炎,王梦颖,柯春亮,陈宇丰   

  1. 中国热带农业科学院生物技术研究所,,,,,,
  • 收稿日期:2014-04-08 修回日期:2014-04-08 接受日期:2014-05-27 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 胡一凤
  • 基金资助:
    中国热带农业科学院院本级基本科研业务费专项“不同硝铵比对香蕉根际微生物群落多样性的影响”（1630052014011）；现代农业产业技 术体系建设专项“国家香蕉产业技术体系栽培技术与耕作岗位”（CARS-32-06）

Colonization of NJQG-3A1 Strain of Endophytic actinomycetes and Its Control Effect on Banana Fusarium Wilt

  • Received:2014-04-08 Revised:2014-04-08 Accepted:2014-05-27 Online:2014-10-15 Published:2014-10-15

摘要： 为明确生防菌NJQG-3A1菌株在香蕉植株根、球茎、假茎、叶和根际土壤中的定殖情况和对香蕉枯萎病的生防作用，为香蕉枯萎病的生防治理提供理论依据，采用抗生素标记法测定菌株NJQG-3A1的定殖能力，以盆栽试验测定其对香蕉枯萎病的防控效果。定殖测定结果表明：以重铬酸钾（100μg/mL）-氨苄青霉素（100μg/mL）标记的NJQG-3A1菌株，可在香蕉根际土壤和植株的根、球茎、假茎中定殖，定殖量表现为：根际土壤＞根＞球茎＞假茎＞叶；在土壤中的定殖量可达1×105 cfu/g；在香蕉植株内定殖量为1×102~1×103 cfu/g。盆栽试验结果表明：经NJQG-3A1菌株的发酵液处理后，香蕉植株的株高、茎围、鲜重比对照（接种病原菌，只施用清水）分别提高了99.22%，44.60%，218.67%，与对照差异显著（P＞0.05）；同时，菌株NJQG-3A1对香蕉枯萎病的防控效果显著，叶部与根茎部的病情指数分别为15、10；病情防效为81.82%，88.98%。NJQG-3A1菌株可以在香蕉植株和根际土壤中定殖，且该菌株的发酵液对香蕉枯萎病具有良好的防控效果，对香蕉植株的生长具有促进作用。

关键词: 种植, 种植

Abstract: The colonization capacity of NJQG- 3A1 strain in banana plants and the rhizosphere was investigated, and the control efficacy of NJQG-3A1 against banana fusarium wilt was also studied, which will provide a theoretical basis for ecological control of the disease. The colonization trends of NJQG- 3A1 in banana plants and the rhizosphere was examined with the method of antibiotic-resistance. Furthermore, the effect of NJQG-3A1 against banana fusarium wilt was investigated by using pot experiment method. Results showed that NJQG- 3A1, tagged by the resistance of‘Ampicllin (100μg/mL)- Potassium dichromate (100μg/mL)’, could colonized in roots, corms and pesudostems tissue of banana and rhizosphere soil stably. The population of NJQG-3A1 in different part was: rhizosphere soil＞roots＞corms＞pesudostems＞leaves. It was also found that the population colonied in the rhizosphere and the banana plant was 1×105 cfμ/g, 1×102 to 1×103 cfμ/g respectively. In addition, the pot experiment result showed that the banana plant height, steam circumference, fresh weight were increased by 99.22%, 44.60%, 218.67% respectively, significantly different from the control (P＜0.05), when treated with the strain NJQG-3A1 fermentation liquid strain NJQG-3A1 also showed obvious biocontrol effect on banana banana fusarium wilt, the disease indexes of leaves and root-steam were 15, 10 respectively, and the control efficacy were 81.82% , 88.98% , respectively. NJQG- 3A1 could colonize in the banana plant and the rhizosphere. The strain NJQG- 3A1 fermentation liquid showed strong disease biocontrol effect on banana fusarium wilt, as well as promotion effect on banana plant growth.