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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (9): 194-199.doi: 10.11924/j.issn.1000-6850.casb14110048

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

紫外辐射对普陀水仙生理生化指标的影响

马玉心崔大练   

  1. 浙江海洋学院海洋科学与技术学院
  • 收稿日期:2014-11-09 修回日期:2015-01-21 接受日期:2015-01-23 出版日期:2015-04-07 发布日期:2015-04-07
  • 通讯作者: 马玉心崔大练
  • 基金资助:
    海洋科学浙江省重中之重学科开放课题“无人海岛植被多样性季节调查与开发利用”(111040602136)；浙江省大学生科技创新活动计划暨新苗人才计划项目“盐地植物开发与利用”（2014R411052）

Effects of UV Radiation on the Physiological and Biochemical Indexes of Narcissus tazetta Linn.var. chinensis

  • Received:2014-11-09 Revised:2015-01-21 Accepted:2015-01-23 Online:2015-04-07 Published:2015-04-07

摘要： 研究紫外线辐射下普陀水仙茎、叶生理生化指标的变化，为进一步研究开发利用海岛植物提供理论依据。本实验紫外辐射时间共设3个梯度，2h、12h、24h，以0h为对照组，测定了普陀水仙茎、叶中SOD、POD、MDA、脯氨酸、电导率、可溶性蛋白质、可溶性糖及叶绿素等生理生化指标。结果表明：随着紫外辐射时间延长，茎、叶中SOD和POD活性变化呈先上升后下降趋势，辐射时间为2h时达到最高，茎中的POD活性明显低于叶。茎和叶中脯氨酸、可溶性糖、MDA含量和电导率均随着紫外辐射时间延长而增大。茎、叶中可溶性蛋白质的含量呈先上升后下降趋势，辐射时间为12h时达到最大。叶绿素a、叶绿素b和类胡萝卜素的含量均呈先增高后减少的趋势，叶绿素的含量在辐射时间2h时达到最大。综合研究认为，紫外辐射下2-12h之间，普陀水仙茎、叶具较强的抗紫外辐射特性，自身能够有效地解除紫外辐射对其造成的伤害。

关键词: 生育期, 生育期

Abstract: This paper studied the changes under UV radiation of physiological and biochemical indexes in the N. tazetta Linn.var.chinensis stems and leaves, and provided a theoretical basis for the further development and utilization of island plants. In this experiment, the time of UV irradiation set up on the 3 gradient, 2, 12, 24h, 0h as control group. We were measured the SOD, POD, MDA, proline, electric conductivity, soluble protein, soluble sugar and chlorophyll other physiological and biochemical indexes of N. tazetta Linn.var.chinensis stems and leaves. The results showed that: with the time of UV-B expanding, the activity of SOD and POD in both stems and leaves were rise at first, and then drop, when the time up to 2 h, it the activity reached the highest. The activity of POD in stems is lower than leaves significantly. The proline, soluble sugar, MDA concentration and conductivity of Stems and leaves increased with prolonged UV radiation time. The contents of soluble protein in both leaves and stems were rise at first and then drop, the highest when the effect time for 12 h. Chlorophyll a, chlorophyll b and carotenoid were rise at first, and then rise, when the time up to 2 h, the contents of Chlorophyll a reached the highest. A comprehensive study that, under the ultraviolet radiation in 2~12h between, N. tazetta Linn.var.chinensis stems and leaves had stronger anti UV radiation characteristic, It could effectively relieve the ultraviolet radiation on the damage by itself.