大孔吸附树脂分离纯化多穗石柯根皮苷

doi:10.11924/j.issn.1000-6850.casb15080143

中国农学通报 ›› 2015, Vol. 31 ›› Issue (36): 269-275.doi: 10.11924/j.issn.1000-6850.casb15080143

大孔吸附树脂分离纯化多穗石柯根皮苷

秦昱¹,丰金玉¹,段继春²,龚志华^1,3,肖文军^1,3

(¹湖南农业大学国家植物功能成分利用工程技术研究中心,长沙 410128；²长沙德康生物科技有限公司,长沙 410100；³湖南省植物功能成分利用协同创新中心,长沙 410128）

收稿日期:2015-08-28 修回日期:2015-11-30 接受日期:2015-10-20 出版日期:2015-12-30 发布日期:2015-12-30
通讯作者: 肖文军
基金资助:
长沙市科技重点项目“多穗石柯根皮苷高纯品生产技术研究与开发”(K1401050-21)；湖南省科技项目“多穗石柯植物中根皮苷高效提制技术合作研究”(2013WK3002)。

Separation and Purification of Phlorizin from Lithocarpus polystachyus Rehd. by Macroporous Resin

Qin Yu¹, Feng Jinyu¹, Duan Jichun², Gong Zhihua^1,3, Xiao Wenjun^1,3

(¹National Research Center of Engineering Technology for Utilization of Botanical Ingredients, Hunan Agricultural University, Changsha 410128; ²Dekang Biotechnology Limited Company, Changsha 410100; ³Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128)

Received:2015-08-28 Revised:2015-11-30 Accepted:2015-10-20 Online:2015-12-30 Published:2015-12-30

摘要/Abstract

摘要： 为优化筛选最优树脂分离纯化根皮苷的工艺技术参数，以多穗石柯根皮苷提取液为原料，比较研究D101、X-5、ADS-7、S-8、AB-8、NKA-9、HPD-100及HPD-400 8种大孔吸附树脂对多穗石柯根皮苷的静态吸附与解吸效果。结果表明：S-8树脂对根皮苷有较好的吸附与解吸效果，静态吸附量与解吸量分别达到14.70 mg/g和8.395 mg/g。当主要考虑根皮苷得率时，最优工艺条件为根皮苷上样浓度 0.9 mg/mL、上样流速3 BV/h、上样体积7 BV，洗脱剂乙醇体积分数60%、洗脱流速为4 BV/h、洗脱体积为3 BV，其根皮苷得率为89.89%，纯度为10.50%，根皮苷分离富集倍数为2.76倍；当主要考虑根皮苷纯度时，最优工艺参数为根皮苷上样浓度0.9 mg/mL、上样流速3 BV/h、上样体积7 BV，洗脱剂乙醇体积分数70%、洗脱流速为3 BV/h、洗脱体积为3.5 BV，其根皮苷纯度为13.51%，得率为83.26%，根皮苷分离富集倍数为3.56倍。

关键词: 近红外光谱技术, 近红外光谱技术, 天然牧草, 营养价值评价, 应用

Abstract: To optimize the technical parameters for separating phlorizin from Lithocarpus polystachyus Rehd., phlorizin extracting solution of Lithocarpus polystachyus Rehd. was used as material, static adsorption and desorption capacities of eight types of macroporous resin including D101, X-5, ADS-7, S-8, AB-8, NKA-9, HPD-100 and HPD-400 to phlorizin were analyzed. The results showed that S-8 had greater adsorption and desorption capacities to phlorizin, which was 14.70 mg/g and 8.395 mg/g, respectively. When considering the maximum phlorizin yield, the optimal concentration, flow rate and volume of phlorizin solution was determined to be 0.9 mg/mL, 3 BV/h and 7 BV, respectively; the optimal concentration, flow rate and volume of the eluent was determined to be 60%, 4 BV/h and 3 BV, respectively, and the yield was 89.89%, which was 1.76 times more than the original solution, while the purity rate was 10.50%. Whereas when considering the maximum phlorizin purity, the optimal concentration, flow rate and volume of phlorizin solution was determined to be 0.9 mg/mL, 3 BV/h, 7 BV, respectively; the optimal concentration, flow rate, and volume of the eluent was determined to be 70%, 3 BV/h and 3.5 BV, respectively, and the purity rate was 13.51%, which was 2.56 times more than the original solution, while the yield was 83.26%.

中图分类号:

TS201

秦昱,丰金玉,段继春,龚志华,肖文军. 大孔吸附树脂分离纯化多穗石柯根皮苷[J]. 中国农学通报, 2015, 31(36): 269-275.

Qin Yu,Feng Jinyu,Duan Jichun,Gong Zhihua and Xiao Wenjun. Separation and Purification of Phlorizin from Lithocarpus polystachyus Rehd. by Macroporous Resin[J]. Chinese Agricultural Science Bulletin, 2015, 31(36): 269-275.

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大孔吸附树脂分离纯化多穗石柯根皮苷

Separation and Purification of Phlorizin from Lithocarpus polystachyus Rehd. by Macroporous Resin

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