GlyCAM-1在人工诱导发情绵羊子宫的表达研究

doi:10.11924/j.issn.1000-6850.casb16010024

中国农学通报 ›› 2016, Vol. 32 ›› Issue (14): 13-16.doi: 10.11924/j.issn.1000-6850.casb16010024

所属专题：畜牧兽医

GlyCAM-1在人工诱导发情绵羊子宫的表达研究

熊善辉,倪和民,齐晓龙,常迪,朱伯阳,盛熙晖,刘云海,郭勇

(北京农学院动物科学技术学院,北京102206）

收稿日期:2016-01-07 修回日期:2016-01-21 接受日期:2016-01-25 出版日期:2016-05-17 发布日期:2016-05-17
通讯作者: 郭勇
基金资助:
项目基金：2015年度国家自然基金项目“MUC-1协调GlyCAM-1调控绵羊子宫容受态的研究”(31470118)；2013年北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(IDHT20130515)。

Expression of GlyCAM-1 in Uterus of Artificially Induced Oestrous Sheep

Xiong Shanhui, Ni Hemin, Qi Xiaolong, Chang Di, Zhu Boyang, Sheng Xihui, Liu Yunhai, Guo Yong

(College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206)

Received:2016-01-07 Revised:2016-01-21 Accepted:2016-01-25 Online:2016-05-17 Published:2016-05-17

摘要/Abstract

摘要： 为探讨GlyCAM-1在人工诱导发情绵羊子宫组织中的表达变化，应用荧光实时定量PCR和蛋白免疫印迹法检测在绵羊发情后第10、12、14、16、18天子宫组织中GlyCAM-1的表达情况。荧光实时定量PCR结果显示：GlyCAM-1 mRNA表达量在第10~18天呈现先减少后增加的趋势，其中绵羊发情后第10天GlyCAM-1 mRNA的相对表达量最大，显著高于其他4个时间点(P<0.05)，第12天显著降低 (P<0.05)，之后相对保持恒定，到第18天又显著升高(P<0.05)。Western blot检测结果证实，GlyCAM-1蛋白约34 kD，子宫组织中GlyCAM-1蛋白表达量在第10~18天亦呈现先逐渐减少然后又逐渐增加的波动趋势，其中发情第10天蛋白表达量最多，显著高于其他4个时间点(P<0.05)，第12、14、16天GlyCAM-1的表达量降低但变化不明显(P>0.05)，第18天表达量显著升高(P<0.05)。在绵羊发情后第10~18天，GlyCAM-1在子宫的转录、表达呈现先减少后增加的波动变化趋势。

关键词: 苹果, 苹果, 矮砧, 矮化, 花果, 分离变异

Abstract: To investigate the changes in the expression of GlyCAM-1 in uterus of oestrous sheep which was artificially induced, expression changes of GlyCAM-1 on the 10^th, 12^th, 14^th, 16^th and 18^th day after sheep oestrus were detected respectively by Real-Time PCR and Western blot. The results of Real-Time PCR showed that mRNA of sheep GlyCAM-1 increased on the 10^th day, decreased on the 14^th and 16^th day, but increased again on the 18^th day, which were in accordance with the results of protein expression detected by Western blot. Protein of 34 kD was detected by this method with specific antiserum. The transcriptional and expressional level of GlyCAM-1 on the 10^th day was significantly higher than that of the other days (P<0.05). In addition, GlyCAM-1 mRNA expression on the 12^th day reduced significantly (P<0.05), and then remained relatively constant, but significantly increased on the 18^th day. There were no significant difference of protein expression among the 12^th, 14^th and 16^th day (P>0.05), but there were significant differences among the other days (P<0.05). Protein expression of the 18^th day increased significantly (P<0.05). Thus it could be concluded that during the 10^th-18^th day after sheep oestrus, the transcriptional and expressional level of GlyCAM-1 presented a decrease-increase trend.

熊善辉,倪和民,齐晓龙,常迪,朱伯阳,盛熙晖,刘云海,郭勇. GlyCAM-1在人工诱导发情绵羊子宫的表达研究[J]. 中国农学通报, 2016, 32(14): 13-16.

Xiong Shanhui,Ni Hemin,Qi Xiaolong,Chang Di,Zhu Boyang,Sheng Xihui,Liu Yunhai and Guo Yong. Expression of GlyCAM-1 in Uterus of Artificially Induced Oestrous Sheep[J]. Chinese Agricultural Science Bulletin, 2016, 32(14): 13-16.

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GlyCAM-1在人工诱导发情绵羊子宫的表达研究

Expression of GlyCAM-1 in Uterus of Artificially Induced Oestrous Sheep

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