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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (27): 109-113.doi: 10.11924/j.issn.1000-6850.casb16010044

• 生物技术科学 • 上一篇    下一篇

高速逆流色谱法分离澳洲薄荷中香叶木苷

刘雪辉,谢红旗,张 思,丁逸凡,李 坚,张志旭,刘东波   

  1. 湖南农业大学园艺园林学院药用植物资源工程系,湖南农业大学园艺园林学院中药资源与开发系,湖南农业大学园艺园林学院药用植物资源工程系,湖南农业大学园艺园林学院中药资源与开发系,国家中医药管理局亚健康干预技术实验室,国家中医药管理局亚健康干预技术实验室,国家中医药管理局亚健康干预技术实验室
  • 收稿日期:2016-01-11 修回日期:2016-02-23 接受日期:2016-03-24 出版日期:2016-09-29 发布日期:2016-09-29
  • 通讯作者: 刘东波
  • 基金资助:
    国家国际科技合作专项项目“澳大利亚特色植物引进与利用关键技术合作研究”(2013DFA31790)。

Separation and Preparation of Diosmin from Mentha×gracilis by High Speed Counter-current Chromatograph High Speed Counter-current Chromatograph

刘雪辉,,,,, and 刘东波   

  • Received:2016-01-11 Revised:2016-02-23 Accepted:2016-03-24 Online:2016-09-29 Published:2016-09-29

PDF (PC)

414

摘要： 为研究澳洲薄荷中香叶木苷的高效制备问题，以澳洲薄荷为原料，建立高速逆流色谱仪分离澳洲薄荷中香叶木苷的方法。澳洲薄荷干燥茎叶用65%乙醇提取，经AB-8 型大孔吸附树脂初步纯化后得到澳洲薄荷总黄酮提取物，然后采用乙酸乙酯-正丁醇-水-乙酸(3:1:3:0.005，V/V)的两相溶剂体系进行香叶木苷单体的高速逆流分离纯化：上相为固定相，下相为流动相，主机转速1600 r/min，流动相流速0.8 mL/min，柱温25℃，检测波长254 nm。分离得到的香叶木苷经液相色谱检测纯度达95.2%。

关键词: 软枣猕猴桃, 软枣猕猴桃, 种质资源, 多样性, 相关性, 主成分分析

Abstract: To investigate a suitable method for preparation of diosmin from Mentha × gracilis. The stems and leaves of Mentha × gracilis was extracted with 65% ethanol and purificated through AB-8 macroporous resin. A solvent system of ethyl acetate-n-butanol-water-acetic acid (3:1:3:0.005, V/V) was used for isolation and preparation by HSCCC (high speed counter-current chromatography). The upper phase was used as the stationary phase, while the lower one was used as mobile phase. The optimum purification conditions were as follow: isolation was performed at a flow-rate of 0.8 mL/min under rotator speed of 1600 r/min. Temperature was 25℃ and detection wavelength was 254 nm. Fraction II were collected according to HSCCC chromatogram and then detected by HPLC, which was diosmin with the purity of 95.2%.

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