甘露聚糖酶协同水解甘露聚糖研究进展

doi:10.11924/j.issn.1000-6850.casb16110041

中国农学通报 ›› 2017, Vol. 33 ›› Issue (21): 21-26.doi: 10.11924/j.issn.1000-6850.casb16110041

所属专题：生物技术

甘露聚糖酶协同水解甘露聚糖研究进展

王瑶¹,王睿琪¹,那金¹,郭尚旭¹,赵丹^1,2

(¹黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨 150080；²黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500）

收稿日期:2016-11-08 修回日期:2017-07-06 接受日期:2016-12-23 出版日期:2017-07-27 发布日期:2017-07-27
通讯作者: 王瑶
基金资助:
国家自然科学基金青年科学基金项目“酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制”(31300355)；哈尔滨市科技局青年后备人才项目“功能性乳酸菌发酵酸菜生态学过程研究”(2014RFQXJ101)；黑龙江省政府博士后项目“疣孢漆斑菌(Myrothecium verrucaria)NF-05漆酶的产生、特性及应用研究”(LBH-Z15214)；黑龙江大学杰出青年基金项目“果胶质降解菌在温水沤麻生态系统中的过程与功能研究”(JCL201305) ；黑龙江大学大学生创新创业训练计划项目“细菌甘露聚糖酶对魔芋葡甘聚糖降解效率的研究”(2016323)。

The Synergistic Enzymatic Hydrolysis of Mannans by Mannanases

Wang Yao¹, Wang Ruiqi¹, Na Jin¹, Guo Shangxu¹, Zhao Dan^1,2

(¹Key Laboratory of Microbiology, Life Science College, Heilongjiang University, Harbin 150080;²Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500)

Received:2016-11-08 Revised:2017-07-06 Accepted:2016-12-23 Online:2017-07-27 Published:2017-07-27

摘要/Abstract

摘要： 甘露聚糖是软质木材中的重要多糖。不同植物来源的甘露聚糖在成分、结构和复杂性上差异很大。甘露聚糖水解为单糖需要甘露聚糖酶、甘露糖苷酶、半乳糖苷酶等多种酶的协同作用，但研究其两者或三者之间的作用较少。为了进一步探究甘露聚糖酶协同水解甘露聚糖的作用机制，本文在概述甘露聚糖来源及结构的基础上，重点论述了甘露聚糖酶的来源、种类、作用方式及产物类型，尤其是通过多种甘露聚糖酶的协同作用增加甘露聚糖的水解效率。最后，展望了甘露聚糖酶协同作用进程与机理的研究方向，为甘露聚糖酶开发利用提供基础。

关键词: 果蝇, 果蝇, Cry1Ab蛋白, 保护酶, 生长

Abstract: Mannan is an important polysaccharide found in softwoods. Mannans from various sources display large differences in composition, structure and complexity. To hydrolysis mannans into monomer sugars requires a number of enzymes like mannanases, mannosidases and galactosidases working in synergy, but several studies have investigated the effect of the two or three. In order to explore the mechanism of mannanase synergistic hydrolysis of mannan, this paper stated mannans’ sources and its structure firstly. Then the sources, species, action mode of mannanase and product type were summarzied, especially the high efficiency of the synergistic enzymatic hydrolysis of mannans by mannanase. Finally, this paper prospected the research direction of process and mechanism of mannanase’s synergistic reaction, and provided the basis for the development and utilization of mannanase.

中图分类号:

王瑶,王睿琪,那金,郭尚旭,赵丹. 甘露聚糖酶协同水解甘露聚糖研究进展[J]. 中国农学通报, 2017, 33(21): 21-26.

Wang Yao,Wang Ruiqi,Na Jin,Guo Shangxu and Zhao Dan. The Synergistic Enzymatic Hydrolysis of Mannans by Mannanases[J]. Chinese Agricultural Science Bulletin, 2017, 33(21): 21-26.

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甘露聚糖酶协同水解甘露聚糖研究进展

The Synergistic Enzymatic Hydrolysis of Mannans by Mannanases

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