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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (1): 61-67.doi: 10.11924/j.issn.1000-6850.casb16110114

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

根际高温对不同砧木黄瓜嫁接幼苗生长、 光合及生理特性的影响

李思思,张红梅,金海军,丁小涛,余纪柱,朱月林   

  1. 上海市农业科学院设施园艺研究所 上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所 上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所 上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所 上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所 上海市设施园艺技术重点实验室,南京农业大学园艺学院
  • 收稿日期:2016-11-22 修回日期:2016-12-25 接受日期:2017-01-10 出版日期:2018-01-12 发布日期:2018-01-12
  • 通讯作者: 余纪柱
  • 基金资助:
    公益性行业(农业)科研专项“蔬菜现代产业技术体系研究与建立”(nyhyzx07-007)；上海市科研计划项目“优质抗病黄瓜种质资源创新及新品种选育”(16391901100)；上海市科技兴农推广项目“基于互联网 的智慧农场示范”(沪农科推字(2016)第2-5-8号)。

Effects of Rhizosphere High Temperature on Growth, Photosynthesis and Biochemical Indexes of Grafted Cucumber Seedlings on Different Rootstocks

  • Received:2016-11-22 Revised:2016-12-25 Accepted:2017-01-10 Online:2018-01-12 Published:2018-01-12

摘要： 为研究嫁接提高黄瓜耐热性的问题，以黑籽南瓜(Cucurbita ficifolia)、日本南瓜(Cucurbita moschata)、‘甬砧8 号’白籽南瓜(Cucurbita maxima× Cucurbita moschata‘Yongzhen No.8’)、‘五叶香’丝瓜(Luffa cylindrica‘Wuyexiang’)和‘傲美’苦瓜(Momordica charantin‘Aomei’)为砧木，‘春秋王2 号’黄瓜(Cucumis sativus‘Chunqiuwang No.2’)为接穗，采用营养液栽培法，研究25℃(CK)和35℃根际温度下处理5 天及恢复生长5 天后不同砧木黄瓜嫁接苗和自根苗的生长、光合及生理特性变化。结果表明，35℃根际高温处理后，不同嫁接苗和自根苗的株高、茎粗、叶片数和叶面积都有所增长，其中‘五叶香’丝瓜砧木嫁接苗生长量最大，且‘五叶香’丝瓜砧木嫁接苗的叶片净光合速率(Pn)、抗氧化酶活性和脯氨酸含量都显著高于其他几种嫁接苗及自根苗，同时，丙二醛(MDA)含量最低。‘五叶香’丝瓜作为砧木嫁接黄瓜能更好地提高其对根际高温的耐受性，为黄瓜的耐热性研究提供理论依据。

关键词: 葡萄, 葡萄, SSR标记, 亲缘关系, 聚类分析

Abstract: This study aims at improving cucumber heat tolerance through grafting. By hydroponic cultivation, the authors took Cucurbita ficifolia, Cucurbita moschata, Cucurbita maxima × Cucurbita moschata‘Yongzhen No.8’, Luffa cylindrica‘Wuyexiang’and Momordica charantin‘Aomei’as rootstocks, and Cucumis sativus ‘Chunqiuwang‘No.2’as scion, to analyze the change of growth, photosynthesis and biochemical indexes of cucumber seedlings treated by normal temperature (25℃) and high temperature (35℃) in rhizosphere for 5 d and recovered for 5 d. The results showed that grafted cucumber seedlings on different rootstocks had different plant height, stem diameter, blade number and leaf area under high temperature stress in rhizosphere, the increment of grafted seedling of Luffa cylindrica‘Wuyexiang’was the largest, and its leaf net photosynthetic rate (Pn), antioxidant enzyme activity and proline content were significantly higher than other kinds of grafting seedlings, at the same time, its malondialdehyde (MDA) content was the lowest. It is suggested that Luffa cylindrica‘Wuyexiang’taken as rootstock for grafting cucumber could improve the tolerance of rhizosphere high temperature, and provide a theoretical basis for studying cucumber heat resistance.