增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响

doi:10.11924/j.issn.1000-6850.casb17010077

中国农学通报 ›› 2018, Vol. 34 ›› Issue (4): 76-82.doi: 10.11924/j.issn.1000-6850.casb17010077

所属专题：生物技术；园艺

增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响

张玉红,陈路瑶,刘彤,高欣,张锡国

东北林业大学森林植物生态学教育部重点实验室,东北林业大学森林植物生态学教育部重点实验室,东北林业大学林学院,东北林业大学森林植物生态学教育部重点实验室,东北林业大学森林植物生态学教育部重点实验室

收稿日期:2017-01-16 修回日期:2018-01-31 接受日期:2017-02-25 出版日期:2018-02-26 发布日期:2018-02-26
通讯作者: 张玉红
基金资助:
国家公益性行业科研专项经费项目“黄檗高效资源培育与活性成分提取技术研究”(201104066)；国家自然科学基金“黄檗种子休眠解除的生理与蛋白质组响应”(31370630)。

The growth and photosynthetic physiological responses of Phellodendron amurense seedlings under supplementary UV-B radiation

Received:2017-01-16 Revised:2018-01-31 Accepted:2017-02-25 Online:2018-02-26 Published:2018-02-26

摘要/Abstract

摘要： [目的]为了研究紫外辐射对药用植物生长和光合生理影响机制,[方法]通过设置三个实验处理对黄檗(Phellodendron amurense)幼苗进行40d的增补UV-B辐射处理,对照组(CK组)自然光照、T1组为自然光照下增补UV-B辐射强度为3.26 μw.cm_-2.nm_-1,T2组为自然光照下增补UV-B辐射强度为9.78 μw.cm_-2.nm_-1。[结果]结果表明黄檗幼苗在增补UV-B辐射处理后,与对照相比,不同辐射强度下单株生物量增长量显著低于对照组(P<0.05),而根冠比则显著降低(P<0.05),并且二者随辐射强度增大均减小；光合速率、蒸腾速率和气孔导度均显著低于对照(P<0.05),并随辐射强度升高而降低；叶绿素初始荧光(F₀)、最大荧光(Fm)、PSⅡ原初光能转换效率(Fv/Fm)、PSⅡ潜在光化学活性(F_V/F₀)、光化学猝灭系数(qP)和表观电子传递速率(ETR),均随UV-B辐射强度增大而降低(P<0.05),非光化学猝灭系数(qN)在低UV-B辐射强度下升高,随辐射强度增大而又降低,但仍高于对照组(P<0.05)。叶绿素a、叶绿素b、总叶绿素、类胡萝卜素和叶绿素a/b等光合色素含量均随增补UV-B辐射强度增大而降低。[结论]说明UV-B辐射处理抑制了黄檗幼苗的生长,降低了光合色素的含量和PSⅡ反应中心传递电子的能力,导致光合作用强度下降,并且上述作用均具有剂量效应。

关键词: 苜蓿, 苜蓿, 微量元素, 结荚率, 种子产量

Abstract: To study the ultraviolet radiation effect on growth and photosynthetic physiological mechanism of medicinal plants, treatments were set to supplement UV-B radiation for 40 days onPhellodendron amurense seedlings, the control treatment (CK) was natural sun light, T1 treatment supplemented UV- B radiation intensity of 3.26 μW/cm2, T2 treatment supplemented UV-B radiation intensity of 9.78 μW/cm2. The results showed that: compared with the control treatment, plant biom ss growth and root shoot ratio were significantly lower afterP. amurense seedlings were exposed under different UVB radiation intensities (P <0.05), and both were reduced with the increase of radiation intensity. Photosynthetic rate, transpiration rate and stomatal conductance were significantly lower than those of the control (P <0.05), and decreased with the increase of radiation intensity; Initial fluorescence (F 0), maximum fluorescence (Fm ), PSⅡ original light energy conversion efficiency (Fv /Fm ), PSⅡ potential photochemical activity coefficient(Fv /F 0) and photochemical quenching (qP ) and electron transport rate (ETR ), all decreased (P <0.05) with the increase of UV-B radiation intensity, onphotochemical quenching coefficient (qN ) raised at low radiation intensity, but decreased with the radiation intensity increase, and still higher than the control (P <0.05). Photosynthetic pigment content, including chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and chlorophyll a/b, decreased with the increment of supplementary UV- B radiation intensity. UV- B radiation treatment inhibited the seedlings’growth of P. amurense , reduced the content of photosynthetic pigment and transmission electron ability of PSⅡ reaction center, which led to the decrease of the photosynthesis intensity and had dose effect.

中图分类号:

S567.1

张玉红,陈路瑶,刘彤,高欣,张锡国. 增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响[J]. 中国农学通报, 2018, 34(4): 76-82.

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增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响

The growth and photosynthetic physiological responses of Phellodendron amurense seedlings under supplementary UV-B radiation

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