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中国农学通报 ›› 2017, Vol. 33 ›› Issue (17): 32-38.doi: 10.11924/j.issn.1000-6850.casb17030144

所属专题: 生物技术 园艺

• 生物技术科学 • 上一篇    下一篇

辣椒Trihelix转录因子家族的生物信息学分析

张春利1,2,袁 岐1,2,赵婷婷1,2,许向阳1,2   

  1. (1东北农业大学园艺园林学院,哈尔滨 150030;2东北农业大学农业部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨 150030)
  • 收稿日期:2017-03-22 修回日期:2017-05-09 接受日期:2017-04-21 出版日期:2017-06-16 发布日期:2017-06-16
  • 通讯作者: 张春利
  • 基金资助:
    现代农业产业技术体系专项基金“国家大宗蔬菜产业技术体系岗位专家”(CARS-25-A-15);国家自然科学基金“抗叶霉病基因Cf19 的克隆功能验证及防御信号传导途径的研究”(31272171);黑龙江省大宗蔬菜新品种选育项目“黑龙江省大宗蔬菜新品种选育”(GA15B103-1);农业部东北地区园艺作物生物学与种质创制重点实验室开放课题基金项目资助。

Bioinformatics Analysis of Trihelix Transcription Factors from Chilli Pepper

Zhang Chunli1,2, Yuan Qi1,2, Zhao Tingting1,2, Xu Xiangyang1,2   

  1. (1College of Horticulture and Landscape Architecture, Northeast Agricultural University Northeast Agricultural University, Harbin 150030; 2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University Northeast Agricultural University, Harbin 150030)
  • Received:2017-03-22 Revised:2017-05-09 Accepted:2017-04-21 Online:2017-06-16 Published:2017-06-16

摘要: Trihelix转录因子在调控植物生长发育以及响应逆境胁迫等多方面发挥着重要作用,属于一个小家族。通过系统地阐述辣椒Trihelix转录因子家族成员特征和进化关系,进一步为研究辣椒Trihelix转录因子的生物学功能提供理论基础。本研究在辣椒基因组范围内,通过HMMER 3.0软件鉴定Trihelix基因家族成员,应用CDD验证其蛋白的功能结构域;采用MEGA5.2软件进行系统进化树分析;利用MEME和MapInspect工具对其蛋白序列进行基序和染色体定位分析。利用功能已知的拟南芥Trihelix蛋白家族为参考序列,系统分析鉴定了28个辣椒Trihelix家族基因,并将其分为GT-1、GT-2、GTγ、SIP1和SH4 5个亚族。基因定位表明,辣椒Trihelix家族成员不均匀的分布在12条染色体上,其中7号和11号染色体上没有辣椒Trihelix成员的分布。保守元件显示辣椒Trihelix基因家族成员部分是高度保守的,各个家族均具有特殊的结构域。辣椒Trihelix基因家族大部分成员结构是保守的,保守基序与聚类分析具有高度的一致性,有可能参与调控辣椒生长发育及逆境胁迫等多种过程。

关键词: 秋播蚕豆品种, 秋播蚕豆品种, 农艺性状, 相关分析, 主成分分析

Abstract: Abstract: Trihelix transcription factors play a significant role in the regulation of plant growth and development, as well as in response to environmental stresses, and belong to a small family. In order to provide theoretical basis for further study of biology function of pepper Trihelix transcription factors, this paper systematically elaborates the characteristics and phylogenetic relationships of pepper Trihelix transcription factor family. Based on the pepper genome database, pepper Trihelix gene family were identified using HMMER 3.0 software and the functional domain of the protein was verified by CDD. A phylogenetic tree was created using the MEGA5.2 software. Motif and chromosomes locations of protein sequence were done by MEME and MapInspect tools. Identified systematically 28 Trihelix family genes from pepper, then was classified into GT-1、GT-2、GTγ、SIP1 and SH4 five subfamilies according to the known Arabidopsis thaliana Trihelix protein family as reference sequences. Gene mapping analysis showed that pepper Trihelix genes were distributed unevenly on 12 chromosomes, among which there was no distribution of pepper Trihelix members on chromosomes 7 and 11. Conserved components analysis indicated that the part of pepper Trihelix genes family was highly conserved, and each subfamily has a special domain. These results suggested that the majority of pepper Trihelix genes were highly conserved, conservative motif and the cluster analysis have a consistence?of high degree and might participated in the multiple regulation process of growth, development and environmental stress in pepper.