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中国农学通报 ›› 2006, Vol. 22 ›› Issue (4): 203-203.

所属专题: 生物技术

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四棱豆过氧化物酶同工酶的研究

全妙华,陈东明,付 明,姚元枝   

  • 出版日期:2006-04-05 发布日期:2006-04-05

Study on Peroxidase Isoenzyme of Winged Bean

Quan Miaohua, Chen Dongming, Fu Ming, Yao Yuanzhi   

  • Online:2006-04-05 Published:2006-04-05

摘要: 为了阐明过氧化物酶(POD)同工酶在四棱豆中的分布规律,鉴定不同四棱豆品种间的亲缘关系,选育优势杂交亲本,我们采用聚丙烯酰胺凝胶垂直板电泳技术,研究了同一株四棱豆(K0027)不同节位成熟叶片和16个品种的POD同工酶酶谱特征。结果表明:POD同工酶在同一株四棱豆9个不同节位成熟叶片中的酶带数均为10条,并且各酶带相应的Rf值基本相同;而在16个不同四棱豆品种中酶带数为6~11条,并且各酶带的宽窄、颜色深浅等特征存在明显的差异。结合各酶带的相对迁移率(Rf值)和品种间的联合相似系数(S值)分析可知,(1)POD同工酶在同一株四棱豆不同节位成熟叶片中的表达具有高度的稳定性和相似性;(2)POD同工酶在所选的16个品种间存在明显的差异性,其中K0006和K0035、早熟二号,K0021和K0028品种间的S值在0.2~0.4之间,为最小值,即这两组品种间的亲缘关系最远,为优势杂交亲本组合;(3)同时阐明了POD同工酶在四棱豆中的相关分布规律,为植物分类或品种鉴定提供了科学依据,对杂交育种选材具有重要的实践指导意义。

关键词: 畜禽饲料, 畜禽饲料, 安全性, 控制对策

Abstract: We have studied the characteristics of peroxidase isoenzyme which are distributed in the same winged bean’s mature leaves of 9 different nodes and 16 varieties by plumb plate of polyarclamide gel electrophoresis, for clarifying the rules of POD isoenzyme in the winged bean,identifying their blood relations and selecting the superior parents for cross-breeding. The results showed that the numbers of the enzyme belts in the mature leaves of 9 different nodes are 10, their Rf of which is almost identical. But the numbers are from 6 to 11 in the varieties, and the enzyme belts have obvious difference, such as the width, the color depth and so on. According to Rf of the enzyme belts and the union similarity factor (S value) of 16 winged bean varieties, we drew the following conclusions : (1)the distribution of POD isoenzyme had the high stability and the similarity in the same winged bean’s mature leaves of different nodes. (2)POD isoenzymes of the 16 winged bean varieties had obvious difference. The S values of K0006 and K0035 or zao shu 2,K0021 and K0028 were minimum, from 0.2 to 0.4,that was, the blood relations of the two groups were the estrangest from each other.They were superior parents for cross-breeding in the selected varieties. (3)we clarified the related rules of POD isoenzyme in the winged bean. The characteristics would provide us with the scientific basis of classifying the species or identifying the varieties, and have the important guiding significance to the scientific selection in the cross-breeding at the same time.

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