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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (22): 31-37.doi: 10.11924/j.issn.1000-6850.casb20200100058

所属专题： 生物技术； 园艺

• 林学·园艺·园林 • 上一篇    下一篇

不同处理对芹菜种子萌发过程中抗氧化系统及激素水平的影响

何莉芳1,2(), 郭世荣1, 阎君2, 朱为民1,2()   

  1. 1南京农业大学园艺学院,南京 210095
    2上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室,上海 201403
  • 收稿日期:2020-01-17 修回日期:2020-04-28 出版日期:2020-08-05 发布日期:2020-07-22
  • 通讯作者: 朱为民
  • 作者简介:何莉芳,女,1994年出生,江西上饶人,在读硕士,研究方向为设施栽培。通信地址：201403上海市奉贤区金齐路1000号,E-mail: 13097038985@163.com。
  • 基金资助:
    上海市科委农业领域项目“基于高通量表型技术的高芹菜素芹菜种质创新及新品系培育”(19391900800);上海农委产业体系课题“绿叶蔬菜产业技术体系——优质新品种选育与推广专业组”(沪农科产字2020第2-03号);上海崇明区农业科创项目(2019CNKC-09)

Effects of Different Treatments on the Antioxidant System and Hormones During the Germination of Celery Seeds

He Lifang1,2(), Guo Shirong1, Yan Jun2, Zhu Weimin1,2()   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095
    2Horticulture Research Institute, Shanghai Academy of Agricultural Sciences/ Key Laboratory of Protected Horticulture, Shanghai 201403
  • Received:2020-01-17 Revised:2020-04-28 Online:2020-08-05 Published:2020-07-22
  • Contact: Zhu Weimin

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摘要：

为研究芹菜种子的萌发机制,采用不同试剂组合（5 g/L NaOH+10% PEG、10% PEG+500 mg/L壳聚糖、5 g/L NaOH+500 mg/L壳聚糖）和不同温度(18、24、30℃)对芹菜种子进行处理,观察芹菜种子的萌发指标,并探究种子萌发过程中的抗氧化系统及内源激素水平的变化。结果表明,30℃对种子发芽率、发芽势及发芽指数提高具有促进作用,加快种子萌发进程。与蒸馏水对照组相比,试剂组合处理在18、24℃时种子发芽率、发芽势和发芽指数升高,促进种子萌发,18℃时5 g/L NaOH+500 mg/L壳聚糖处理下的发芽势最高,24℃时5 g/L NaOH+10% PEG处理下发芽势达到最大值;30℃时不同试剂组合处理间发芽率、发芽势和发芽指数无明显差异。18、30℃对种子具有一定程度的胁迫作用,种子SOD和CAT活性较24℃条件下有所增加;18℃时MDA含量和脯氨酸含量显著增加;5 g/L NaOH+10% PEG处理能显著降低MDA含量。萌发过程中,芹菜种子内部ABA含量下降,GA3、ZA含量增加。试剂组合及适当高温有利于提高芹菜种子的发芽率、发芽势和发芽指数,促进种子萌发,提高种子萌发整齐度;芹菜种子萌发过程中抗氧化系统和激素水平会积极响应不同的处理条件。

关键词: 芹菜, 种子萌发, 抗氧化系统, 内源激素, 试剂组合, 温度

Abstract:

To study the germination mechanism of celery seeds, different reagent combinations (5 g/L NaOH+ 10% PEG, 10% PEG+ 500 mg/L chitosan, 5 g/L NaOH+ 500 mg/L chitosan) and different temperatures (18, 24, 30℃) were used to process the seeds and to explore the germination index, the changes in antioxidant system and endogenous hormone levels in seed germination. The results showed that 30℃ could promote the germination rate, germination potential and germination index of seeds, and accelerate the germination process of seeds. Compared with the control group of distilled water, the germination rate, germination potential and germination index of the combination treatment increased at 18 and 24℃. Under the 18℃, 5 g/L NaOH+ 500 mg/L chitosan treatment, the potential was the highest, and the germination potential reached the maximum under the treatment of 5 g/L NaOH+ 10% PEG at 24℃; there was no significant difference in germination rate, germination potential and germination index between different reagent combinations at 30℃. 18 and 30℃ had a certain degree of stress on seeds, and the activities of SOD and CAT in seeds increased compared with those under 24℃. The content of MDA and proline significantly increased at 18℃. The content of MDA was significantly reduced by the treatment of 5 g/L NaOH+ 10% PEG reagent combination. During the germination, the content of ABA gradually decreased, and the contents of GA3 and ZA gradually increased in celery seeds. Therefore, the combination of reagents and appropriate high temperature are beneficial to increase the germination rate, germination potential and germination index of celery seeds, and can also improve the germination uniformity. The antioxidant system and hormone levels of celery seeds actively respond to different treatment conditions during the germination process.

Key words: celery, seed germination, antioxidant system, endogenous hormone, reagent combination, temperature

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