防御信号途径在B型烟粉虱取食烟草诱导抗蚜防御中的作用

doi:10.11924/j.issn.1000-6850.casb20191201004

摘要/Abstract

摘要：

B型烟粉虱取食能够诱导烟草产生对烟蚜的抗性反应,为了明确水杨酸和茉莉酸防御信号途径与烟粉虱诱导抗蚜防御之间的关系,采用生化分析、实时定量PCR、蚜虫生物活性测定等方法比较了烟粉虱若虫取食对烟草水杨酸、茉莉酸含量、通路下游防御基因表达水平的影响及外源水杨酸、茉莉酸对烟蚜生长发育的影响。结果显示：烟粉虱侵染能够显著激活水杨酸防御途径,在取食15天后,水杨酸水平较对照升高1.40倍,水杨酸下游防御基因PR-1a及PR-2a分别较对照升高3.22和0.74倍;然而烟粉虱侵染后烟草叶片茉莉酸含量与对照相比无显著变化,JA下游防御基因PI-II和TPI的转录水平分别较对照降低73.91%和56.73%。生测结果显示,外源施用水杨酸对烟蚜的存活率和相对平均生长率具有显著不利影响。烟草叶片施用1 mmol/L水杨酸后,蚜虫的存活率及相对生长率分别较对照降低43.2%和11.54%;然而喷施茉莉酸甲酯对蚜虫的生长发育无不利影响。上述结果表明,水杨酸介导的防御应答在B型烟粉虱取食诱导烟草的抗蚜防御中起重要作用。

关键词: 烟粉虱, 种间竞争, 茉莉酸, 烟蚜, 水杨酸, 诱导防御

Abstract:

Bemisia tabaci infection can induce the tobacco resistance to Myzus persicae. The study aims to investigate the relationship between the defense signaling of salicylic acid (SA) and jasmonic acid (JA) and the resistance to Myzus persicae induced by Bemisia tabaci. The effects of Bemisia tabaci nymphs feeding on SA and JA level and downstream defense genes expressing level were tested, and the effects of exogenous SA and JA on the growth and development of Myzus persicae were analyzed by biochemical analysis and real-time fluorescent quantitative PCR. The results showed that Myzus persicae infection obviously activated SA defense pathway. After feeding 15 d, the SA level was 1.40-fold higher than the control, and the levels of PR-1a and PR-2a were increased by 3.22 times and 0.74 times, respectively. JA level was not obviously affected, while the transcript levels of JA downstream defense genes PI-II and TPI were decreased by 73.91% and 56.73%, respectively. Biochemical analysis revealed that the application of exogenous SA was harmful to the survival and the relative average growth rate of M. persicae. Treating with 1 mmol/L SA, aphid survival and growth rate were decreased by 43.2% and 11.54%, respectively, compared with the control. Methyl jasmonate had no harmful effect on the growth and development of Myzus persicae. In conclusion, SA mediated defense response contributes to the tobacco resistance against M. persicae.

Key words: Bemisia tabaci, interspecific competition, jasmonic acid, Myzus persicae, salicylic acid, induced defense

中图分类号:

S436.429

徐亚楠, 孙夏, 赵海朋, 薛明. 防御信号途径在B型烟粉虱取食烟草诱导抗蚜防御中的作用[J]. 中国农学通报, 2020, 36(36): 93-99.

Xu Yanan, Sun Xia, Zhao Haipeng, Xue Ming. The Function of Defense Signaling Pathways: Tobacco Resistance to Myzus persicae Induced by Bemisia tabaci[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 93-99.

图/表 5

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基因	登录号	引物
PR-1a	X05959.1	F: 5’-GATTGTAACCTCGTACATTCTCATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PR-2a	M59443.1	F: 5’-TAACCTTCCACTCTTAGCCAATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PI-II	Z29537.1	F: 5’-TAGTTTCGTCGCTCATCT-3’ R: 5’-TAGGGTCACATTCCGTAG-3’
TPI	DQ158200.1	F: 5'-TCAGGAGATAGTAAATATGGCTGTTCA-3’ R: 5'-ATCTGCATGTTCCACATTGCTTA-3’
Actin	X69885.1	F:5’-GCTTGCTTACATTGCTCTCGACTAT-3’ R:5’-GATAGAGTTGTATGTAGTCTCGTG-3’

基因	登录号	引物
PR-1a	X05959.1	F: 5’-GATTGTAACCTCGTACATTCTCATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PR-2a	M59443.1	F: 5’-TAACCTTCCACTCTTAGCCAATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PI-II	Z29537.1	F: 5’-TAGTTTCGTCGCTCATCT-3’ R: 5’-TAGGGTCACATTCCGTAG-3’
TPI	DQ158200.1	F: 5'-TCAGGAGATAGTAAATATGGCTGTTCA-3’ R: 5'-ATCTGCATGTTCCACATTGCTTA-3’
Actin	X69885.1	F:5’-GCTTGCTTACATTGCTCTCGACTAT-3’ R:5’-GATAGAGTTGTATGTAGTCTCGTG-3’