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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (6): 224-224.

所属专题： 园艺

• 目次 • 上一篇    下一篇

大蒜不定芽的诱导及其增殖系数的调节

汤青林,王志敏,宋 明,王小佳,王平利,向巧彦   

  • 出版日期:2006-06-05 发布日期:2006-06-05

Study on Multiplying Buds Induction and Adjustment of Propagation Coefficient in Garlic

Tang Qinglin, Wang Zhimin, Song Ming, Wang Xiaojia, Wang Pingli, Xiang Qiaoyan   

  • Online:2006-06-05 Published:2006-06-05

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616

摘要： 为获得白皮大蒜组培繁殖体系，以其茎尖为材料，通过BA和NAA不同激素配比试验研究，结果表明：诱导愈伤组织的最适培养基为：MS+BA 0.2mg/L + NAA 0.5mg/L，诱导率达60%；诱导不定芽的最适培养基为：MS +BA 1.0mg/L + NAA 0.1mg/L，丛生芽繁殖系数高达80%。同时，为建立扩繁最佳体系，分别研究了激素、pH、谷氨酸钠三因素对不定芽增殖系数的调节效果，研究表明：添加0.5mg/L的GA3使增值系数提高到13.8；pH5.8最利于不定芽增值；添加10mg/L谷氨酸钠能在多次继代培养中保持较高的增值系数。

关键词: 功能性食品, 功能性食品, 抗疲劳, 添加剂, 毒理学

Abstract: The experiments about tissue culture of Garlic shoot-tip were made in MS mediums supplemented with different phytohormones. And then the best medium for rapid propagation of Garlic was obtained. The results were as follows: the optimized medium for inducing callus was MS+BA 0.2mg/L+NAA0.5mg/L, and the growth rate of callus was 60%. The suitable medium for inducing adventitious buds was MS +BA1.0 mg/L+NAA 0.1mg/L, and the highest propagatiion rate reached 80%. At the same time, the effects of different phytohormones, pH and glutamate on the propagation coefficient in Garlic have been researched. Then the results indicated: Propagation coefficient is raised to 13.8 in the medium supplemented with 0.5mg/L GA3. And it also can be raised in pH5.8. Propagation coefficient in subculture is remained at high level in 10mg/L glutamate.