环境和食品中阿维菌素类药物残留量检测方法研究进展

doi:10.11924/j.issn.1000-6850.casb2020-0705

摘要/Abstract

摘要：

阿维菌素类药物是一种常用的杀虫剂,现有报道的食品和环境基质样品中阿维菌素残留分析最为常用的检测仪器为液相色谱-串联质谱。基于此,已建立了一系列的不同样基质中多种阿维菌素类药物残留量的检测方法,本研究除了关注常见的多种阿维菌素残留量的测定方法,也关注了食品和环境样品中单种阿维菌素化合物的专一性检测方法。本研究从阿维菌素在食品和环境领域的应用以及赋存状态、前处理方式（提取和净化方法）、检测方法（液相色谱法、酶联免疫法和液相色谱-串联质谱法）及定量方式和基质干扰等方面,综述了2010—2020年不同检测技术在食品和环境样品基质中阿维菌素类化合物残留分析中的研究与应用进展。最后,综述了多残留量检测方法的局限性,未来的关注点以及对阿维菌素残留分析方法的发展趋势。本研究可为开展烟草中阿维菌素类化合物残留分析技术研究提供参考。

关键词: 阿维菌素, 食品, 环境基质样品, 杀虫剂, 残留量, 检测方法

Abstract:

Avermectins is a kind of frequently used pesticide. The most widely used detection technique for the determination of avermectins residues in food and environmental samples is liquid chromatography coupled to tandem mass spectrometry. Based on this, multi-residue analytical methods with selective sample treatment methodologies in different samples have been developed. This review focuses not only on these common multi-residue methods but also on specific single-residue methods for the analysis of avermectin residues in food and environmental samples. This study summarizes analytical methods in the last decade for the determination of avermectins residues in food and environmental samples, including the applications of avermectin and its occurrence state in food and environmental samples, the sample preparation methods (including extraction and clean methods), analytical methods (including HPLC, ELISA and LC-MS/MS) as well as its quantitation mode and methods to reduce matrix interference. Finally, the limitations of multi-residue methods, the future perspectives and the trends for avermectins residues analysis in food and environmental samples are reviewed. The study will be helpful for future research in the field of determination of avermectins residues in tobacco.

Key words: avermectins, food, environmental sample, pesticides, residue, analytical method

中图分类号:

S-01

熊巍, 陶晓秋, 张海燕. 环境和食品中阿维菌素类药物残留量检测方法研究进展[J]. 中国农学通报, 2021, 37(30): 111-120.

Xiong Wei, Tao Xiaoqiu, Zhang Haiyan. Analytical Methods for the Determination of Avermectins Residues in Food and Environmental Samples: A Review[J]. Chinese Agricultural Science Bulletin, 2021, 37(30): 111-120.

图/表 2

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分析物	样品基质	前处理方式	分析仪器	回收率	精密度	检出限	参考文献
AVM, ABA 1b, ABA 1a, EPR, IVM, MOX	牛奶	PT-MIP-SPE	HPLC-UV	around 100%			[43]
ABA, EPR MOX	水、水果	PT-MIP-SPE	HPLC-UV	88.19%~110.54%	0.59%~3.82%		[42]
AVMs	牛肌肉组织	QuEChERS	UPLC-MS/M	satisfactory		0.007~66.715 µg/kg	[64]
AVMs	水产品	改良的QuEChERS	HPLC-MS/M	71.6%~112.8%	4.7%~13.1%	0.075~1.500 µg/kg	[28]
AVMs	动物组织	乙腈甲醇加速溶剂萃取	HPLC-MS/M	>75%			[16]
IVM, DOR, EPR	土壤	在线SPE	UPLC-MS/MS	74%~89%		0.1~0.2 µg/kg	[41]
AVM	水体沉积物	超声微波萃取,SPE净化	HPLC-MS/M	/	2.2%~16.2%	0.18 ng/g	[13]
EPR, AVM, DOR, IVM	奶及奶粉	20%乙醇乙腈,SPE净化	HPLC-FL	89.2%~113%	0.450%~9.73%	0.15~1.5μg/kg	[72]
EPR, AVM, EMMB	石斑鱼	乙腈提取,SPE净化	HPLC-MS/M	74.6%~93.6%	2.3%~10.9%	0.25~2.5 ng/mL	[11]
AVM, IVM	动物源食品	在线SPE HySphere C_(18) HD	HPLC-MS/MS	74.8%~96.5%	<11%	<5.0 μg/kg	[29]
AVMs	水产品	0.2%(v/v)氨化乙腈提取,dis-SPE净化	HPLC-MS/MS	71.6%~112.8%	4.7%~13.1%	0.75~1.5 μg/kg	[28]
AVM	鱼肉	MIP修饰的磁性多壁碳纳米管萃取	UPLC-MS/MS	84.2%~97.0%	1.7%~2.9%	0.075 μg/kg	[44]
AVMs	牛、山羊、猪肉及其肾脏、牛奶	正己烷脱脂,SPA和C18修饰的多壁碳纳米管萃取净化	HPLC-MS/MS	82%~107%	<15%		[23]
AVM	土壤、烟草	乙腈提取,用SPE-Alumina-B柱净化	HPLC	79.2%~93.6%	0.4%~8.9%	0.01~0.1 mg/kg	[73]
AVM	水	分散液相微萃取	HPLC	82.8%~95.0%	3.3%~7.0%	0.1 μg/mL	[50]
EPR, AVM, DOR, IVM	牛奶	乙腈提取,SPE净化	HPLC-MS/MS	66.4%~120%	<20%	0.15 μg/kg	[36]
IVM, ABA, DOR, EPR, MOX	土壤	在线SPE	HPLC-MS/MS	74%~89%	<19%	0.03~0.06 ng/g	[41]
AVM,多环芳烃	土壤	超声辅助萃取,在线SPE	UPLC-MS/MS	88%	4.85%	0.08 ng/g	[74]
AVMS	食品	QuEChERS	HPLC-MS/MS			2.5 μg/kg	[69]
AVM, IVM, ABA, DOR, EPR	牛肝	在线SPE	HPLC-MS/MS	77.4%~98.4%	4.46%~8.03%	1.5 μg/kg	[40]
AVM, IVM, ABA, DOR, EPR	食品	在线SPE	HPLC-MS/MS	71.8%~101.3%	<8.94%	2.02 μg/kg	[39]
AVM, IVM, ABA, DOR	土壤	乙醇改进的CO₂超流体临界萃取	HPLC-MS/MS	82.5%~96.2%	2.1%~7.9%	1.5 μg/kg	[19]
AVM	辣椒干	乙腈提取,乙腈饱和的正己烷去脂,SPE净化	HPLC-FL	90.3%~103.1%	2.31%~6.93%	5 μg/kg	[53]
AVM	稻田土、稻壳、糙米和稻杆	乙腈或乙酸乙酯提取,SPE净化	UPLC-MS/MS	84%~107%	4.7%~13.6%	0.3~3.0 μg/kg,	[14]
AVMS	牛奶	溶剂提取,无需净化	ELISA	95.9%	15.42%	5 ng/mL	[56]
AVM	牛肝、牛肉	甲醇振荡提取	CLIA	95.25%~104.55%	15%	1.463、1.392 ng/kg	[61]
AVB1a			HPLC-qNMR	98.86%	0.34%	0.009 ?mg/mL	[54]
AVB1a			HPLC-qNMR	93%			[32]

分析物	样品基质	前处理方式	分析仪器	回收率	精密度	检出限	参考文献
AVM, ABA 1b, ABA 1a, EPR, IVM, MOX	牛奶	PT-MIP-SPE	HPLC-UV	around 100%			[43]
ABA, EPR MOX	水、水果	PT-MIP-SPE	HPLC-UV	88.19%~110.54%	0.59%~3.82%		[42]
AVMs	牛肌肉组织	QuEChERS	UPLC-MS/M	satisfactory		0.007~66.715 µg/kg	[64]
AVMs	水产品	改良的QuEChERS	HPLC-MS/M	71.6%~112.8%	4.7%~13.1%	0.075~1.500 µg/kg	[28]
AVMs	动物组织	乙腈甲醇加速溶剂萃取	HPLC-MS/M	>75%			[16]
IVM, DOR, EPR	土壤	在线SPE	UPLC-MS/MS	74%~89%		0.1~0.2 µg/kg	[41]
AVM	水体沉积物	超声微波萃取,SPE净化	HPLC-MS/M	/	2.2%~16.2%	0.18 ng/g	[13]
EPR, AVM, DOR, IVM	奶及奶粉	20%乙醇乙腈,SPE净化	HPLC-FL	89.2%~113%	0.450%~9.73%	0.15~1.5μg/kg	[72]
EPR, AVM, EMMB	石斑鱼	乙腈提取,SPE净化	HPLC-MS/M	74.6%~93.6%	2.3%~10.9%	0.25~2.5 ng/mL	[11]
AVM, IVM	动物源食品	在线SPE HySphere C_(18) HD	HPLC-MS/MS	74.8%~96.5%	<11%	<5.0 μg/kg	[29]
AVMs	水产品	0.2%(v/v)氨化乙腈提取,dis-SPE净化	HPLC-MS/MS	71.6%~112.8%	4.7%~13.1%	0.75~1.5 μg/kg	[28]
AVM	鱼肉	MIP修饰的磁性多壁碳纳米管萃取	UPLC-MS/MS	84.2%~97.0%	1.7%~2.9%	0.075 μg/kg	[44]
AVMs	牛、山羊、猪肉及其肾脏、牛奶	正己烷脱脂,SPA和C18修饰的多壁碳纳米管萃取净化	HPLC-MS/MS	82%~107%	<15%		[23]
AVM	土壤、烟草	乙腈提取,用SPE-Alumina-B柱净化	HPLC	79.2%~93.6%	0.4%~8.9%	0.01~0.1 mg/kg	[73]
AVM	水	分散液相微萃取	HPLC	82.8%~95.0%	3.3%~7.0%	0.1 μg/mL	[50]
EPR, AVM, DOR, IVM	牛奶	乙腈提取,SPE净化	HPLC-MS/MS	66.4%~120%	<20%	0.15 μg/kg	[36]
IVM, ABA, DOR, EPR, MOX	土壤	在线SPE	HPLC-MS/MS	74%~89%	<19%	0.03~0.06 ng/g	[41]
AVM,多环芳烃	土壤	超声辅助萃取,在线SPE	UPLC-MS/MS	88%	4.85%	0.08 ng/g	[74]
AVMS	食品	QuEChERS	HPLC-MS/MS			2.5 μg/kg	[69]
AVM, IVM, ABA, DOR, EPR	牛肝	在线SPE	HPLC-MS/MS	77.4%~98.4%	4.46%~8.03%	1.5 μg/kg	[40]
AVM, IVM, ABA, DOR, EPR	食品	在线SPE	HPLC-MS/MS	71.8%~101.3%	<8.94%	2.02 μg/kg	[39]
AVM, IVM, ABA, DOR	土壤	乙醇改进的CO₂超流体临界萃取	HPLC-MS/MS	82.5%~96.2%	2.1%~7.9%	1.5 μg/kg	[19]
AVM	辣椒干	乙腈提取,乙腈饱和的正己烷去脂,SPE净化	HPLC-FL	90.3%~103.1%	2.31%~6.93%	5 μg/kg	[53]
AVM	稻田土、稻壳、糙米和稻杆	乙腈或乙酸乙酯提取,SPE净化	UPLC-MS/MS	84%~107%	4.7%~13.6%	0.3~3.0 μg/kg,	[14]
AVMS	牛奶	溶剂提取,无需净化	ELISA	95.9%	15.42%	5 ng/mL	[56]
AVM	牛肝、牛肉	甲醇振荡提取	CLIA	95.25%~104.55%	15%	1.463、1.392 ng/kg	[61]
AVB1a			HPLC-qNMR	98.86%	0.34%	0.009 ?mg/mL	[54]
AVB1a			HPLC-qNMR	93%			[32]