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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (27): 111-118.doi: 10.11924/j.issn.1000-6850.casb2022-0222

所属专题： 生物技术； 园艺

• 生物科学 • 上一篇    下一篇

甜菜热激蛋白基因BvHSP18.2的克隆和生物信息学分析

张琼1,2(), 王锦霞1,2,3, 孟诗琪1,2, 钟鑫爱1,2, 刘大丽1,2, 兴旺1,2()   

  1. 1.国家甜菜种质中期库,黑龙江大学,哈尔滨 150080
    2.黑龙江大学省高校甜菜遗传育种重点实验室/现代农业与生态环境学院,哈尔滨 150080
    3.黑龙江大学省高校生化与分子生物学重点实验室/生命科学学院,哈尔滨 150080
  • 收稿日期:2022-03-30 修回日期:2022-06-15 出版日期:2022-10-05 发布日期:2022-09-21
  • 通讯作者: 兴旺
  • 作者简介:张琼,女,1996年出生,山西临汾人,研究生在读,研究方向：作物分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学现代农业与生态环境学院,Tel：18435998652,E-mail： 2549362252@qq.com。
  • 基金资助:
    国家作物种质资源库“甜菜分库运行服务”(NCGRC-2021-017);农业农村部“甜菜种质资源的收集、鉴定、编目、繁种与入库(圃)保存”(19210157);农业农村部“普查收集甜菜种质资源鉴定评价与编目入库”(19210911);农业农村部“甜菜种质资源安全保存”(19211031);国家糖料产业技术体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102);黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057);黑龙江省高校基本科研业务费黑龙江大学专项资金项目“能源甜菜BvMPT11基因的Cd污染生物修复应答机制研究”(KJCX201920)

Sugar Beet Heat-shock Protein Gene BvHSP18.2: Cloning and Bioinformatics Analysis

ZHANG Qiong1,2(), WANG Jinxia1,2,3, MENG Shiqi1,2, ZHONG Xin’ai1,2, LIU Dali1,2, XING Wang1,2()   

  1. 1. National Beet Medium-term Gene Bank, Heilongjiang University, Harbin 150080
    2. Key Laboratory of Sugar Beet Genetics and Breeding, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
    3. Key Laboratory of Molecular Biology, School of Life Sciences, Heilongjiang University, Harbin 150080
  • Received:2022-03-30 Revised:2022-06-15 Online:2022-10-05 Published:2022-09-21
  • Contact: XING Wang

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摘要：

旨在通过基因克隆及生物信息学分析,预测甜菜小分子热激蛋白BvHSP18.2 (LOC104903994)的功能。以甜菜‘780016B/12优’为试验材料克隆BvHSP18.2,获得BvHSP18.2基因全长;通过ProtParam tool、SWISS-MODEL、MEGA11等对BvHSP18.2的理化性质、蛋白结构、多序列比对等信息进行分析。RT-PCR扩增得到的BvHSP18.2基因全长为962 bp,编码158个氨基酸,分子式为C928H1466N266O284S6,属亲水性不稳定的小分子热激蛋白家族;该基因编码蛋白含13个磷酸化位点,α螺旋和无规则卷曲等主要构件。进化关系发现BvHSP18.2与藜麦、菠菜的sHSP同源性相似。同时,BvHSP18.2基因启动子区域具有参与防御和应激反应等顺式作用元件,推测其在甜菜生长发育和胁迫应答中发挥重要作用。本结果为进一步开展该基因的功能和遗传调控机制研究奠定了基础。

关键词: 甜菜, 小分子热激蛋白, BvHSP18.2, 基因克隆, 生物信息学分析

Abstract:

The aim of this paper is to predict the function of sugar beet small heat-shock protein BvHSP18.2 (LOC104903994) by gene cloning and bioinformatics analysis. The full length of BvHSP18.2 gene was obtained by cloning BvHSP18.2 from the test material of sugar beet ‘780016B/12 superior’. The physicochemical properties, protein structure and multiple sequence alignment of BvHSP18.2 were analyzed by ProtParam tool, SWISS-MODEL, MEGA11, etc. The full length of BvHSP18.2 gene amplified by RT-PCR was 962 bp, encoding 158 amino acids, with the molecular formula of C928H1466N266O284S6, which was a hydrophilic unstable protein and belonged to the family of small heat-shock protein family. The protein encoded by this gene contained 13 phosphorylation sites, the alpha helix, irregular curl and other main components. The phylogenetic relationship showed that BvHSP18.2 was similar to the sHSP homologs of quinoa and spinach. Meanwhile, the promoter region of BvHSP18.2 gene had cis-acting elements involved in defense and stress response, and was presumed to play an important role in sugar beet growth and development and stress response. This paper can lay a foundation for further studies on the function and genetic regulation mechanism of this gene.

Key words: Beta vulgaris L., small heat-shock protein (sHSP), BvHSP18.2, gene cloning, bioinformatics analysis

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