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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (11): 43-49.doi: 10.11924/j.issn.1000-6850.casb2022-0257

• 林学·园艺·园林 • 上一篇    下一篇

基于HPLC优化北苍术三种药用成分提取及测定方法

李涵秋1(), 杜靖艳1, 满孜拉·吐达吉1, 宋鑫宇1, 田晔林1(), 王建舫2   

  1. 1 北京农学院园林学院，北京 102206
    2 北京农学院兽医学（中医药）北京市重点实验室/动物类国家级实验教学示范中心，北京 102206
  • 收稿日期:2022-03-30 修回日期:2022-06-15 出版日期:2023-04-15 发布日期:2023-04-10
  • 通讯作者: 田晔林，女，1983年出生，湖南溆浦人，副教授，博士，研究方向：乡土植物资源及药用植物。通信地址：102206 北京市昌平区史各庄街道北农路7号 北京农学院，Tel：010-80799157，E-mail：tianyelin@126.com。
  • 作者简介:

    李涵秋，女，1996年出生，新疆昌吉人，硕士，研究方向：林下资源培育与利用。通信地址：102206 北京市昌平区史各庄街道北农路7号 北京农学院，Tel：010-80799157，E-mail：。

  • 基金资助:
    北京农学院学位与研究生教育改革与发展项目“林下经济校外研究生联合培养实践基地建设”(2020YJS017 2020)

Three Medicinal Ingredients of Atractylodes chinensis (DC.) Koidz: Extraction and Determination Based on HPLC

LI Hanqiu1(), DU Jingyan1, MANZILA·Tudaji1, SONG Xinyu1, TIAN Yelin1(), WANG Jianfang2   

  1. 1 School of Landscape Architecture, Beijing University of Agriculture, Beijing 102206
    2 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine/National Demonstration Center for Animal Experimental Education, Beijing University of Agriculture, Beijing 102206
  • Received:2022-03-30 Revised:2022-06-15 Online:2023-04-15 Published:2023-04-10

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摘要：

为了优化北苍术中苍术素、苍术酮、β-桉叶醇的提取过程及HPLC测定方法。分别对北苍术用量、超声时间、超声温度做了测试，并对色谱条件逐一考察。确定提取条件：北苍术0.8 g，40℃超声 60 min。色谱条件：色谱柱(Venusil MP C18 4.6*150 mm，5 μm，150 A)，柱温28℃。以甲醇:水(82:18)为流动相检测苍术素、苍术酮。以乙腈：0.2%碳酸氢钠：10%甲醇(65:25:10)为流动相检测β-桉叶醇。在0.002~1 mg/mL范围内，苍术素、苍术酮、β-桉叶醇峰面积线性关系良好(R2=0.9999~1，n=6)。平均加样回收率为97.7%~102.17%，RSD为1.52%~2.49%，n=6。该方法准确、简便，可用于1~4年生北苍术中苍术素、苍术酮、β-桉叶醇的含量检测。

关键词: 北苍术, HPLC, 苍术素, 苍术酮, β-桉叶醇

Abstract:

To optimize the extraction procedure of atractylodin, atractylone and β-Eudesmol of Atractylodes chinensis (DC.) Koidz and the HPLC analytical method, the sample consumption, ultrasonic time and ultrasonic temperature in the procedure of sample extraction were analyzed, and chromatographic conditions were investigated one by one. The extraction condition was as follows: sample consumption of 0.8 g, and ultrasonication at 40℃ for 60 min. The chromatographic condition was as follows: chromatographic column (Venusil MP C18, 4.6×150 mm, 5 μm, 150 A), and column temperature of 28℃. To detect atractylodin and atractylone, methanol: water (82:18) was used as the mobile phase; to detect β-Eudesmol, acetonitrile: 0.2% sodium bicarbonate: 10% methanol (65:25:10) was used as the mobile phase. In the range of 0.002-1 mg/mL, the peak areas of atractylodin, atractylone and β-Eudesmol had good linear relationships (R2=0.9999-1, n=6). The average recovery ratio was 97.7%-102.17%, RSD was 1.52%-2.49%, n=6. This method is accurate and simple and it could be used for determining the content of atractylodin, atractylone and β-Eudesmol of A.chinensis of 1-4 years old.

Key words: Atractylodes chinensis (DC.) Koidz, HPLC, atractylodin, atractylone, β-Eudesmol