外源添加苯甲酸对紫苏种子发芽和幼苗生长的影响

doi:10.11924/j.issn.1000-6850.casb2023-0178

中国农学通报 ›› 2024, Vol. 40 ›› Issue (7): 72-77.doi: 10.11924/j.issn.1000-6850.casb2023-0178

外源添加苯甲酸对紫苏种子发芽和幼苗生长的影响

薛彤彤¹(), 谢萌蒙², 孙辉², 李冲伟¹^,²()

¹ 黑龙江大学生命科学学院/农业微生物技术教育部工程研究中心/黑龙江省寒地生态修复与资源利用重点实验室/黑龙江省普通高校微生物重点实验室，哈尔滨 150080
² 桦南农盛园食品有限公司，黑龙江桦南 154400

收稿日期:2023-03-03 修回日期:2023-09-06 出版日期:2024-02-27 发布日期:2024-02-27
通讯作者:
李冲伟，男，1977年出生，教授，博士，研究方向：微生物生态学。通信地址：150500 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学，E-mail：chongweili@126.com。
作者简介:
薛彤彤，女，1997年出生，硕士研究生，研究方向：生态学。通信地址：150500 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学，E-mail：3465255355@qq.com。
基金资助:
黑龙江省自然科学基金项目“寒区农业土壤面源污染微生物调控技术的研究”(TD2019C002)

Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens

XUE Tongtong¹(), XIE Mengmeng², SUN Hui², LI Chongwei¹^,²()

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/ Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/ Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080
² Huanan Nongshengyuan Food Co., Ltd., Huanan, Heilongjiang 154400

Received:2023-03-03 Revised:2023-09-06 Published-:2024-02-27 Online:2024-02-27

摘要/Abstract

摘要：

为探究外源苯甲酸胁迫对紫苏种子萌发、幼苗生长及叶片抗氧化酶活性的影响，添加苯甲酸对紫苏种子进行培养皿滤纸培养和室内盆栽实验，设置0、1、1.5、2、2.5、3 mmol/L 6个梯度处理，研究不同浓度苯甲酸胁迫对紫苏种子发芽率、发芽势、发芽指数、活力指数、紫苏幼苗生长以及抗氧化酶活性的影响。结果表明，随着苯甲酸浓度的增加，紫苏种子的发芽率、发芽势、发芽指数、活力指数总体表现为低浓度促进、高浓度抑制；紫苏幼苗的根重、胚根长、胚轴长和株鲜重均呈显著下降趋势，幼苗叶片过氧化氢酶和过氧化物酶活性呈显著下降趋势(P<0.05)，超氧化物歧化酶活性呈先升高后下降趋势，在1.5 mmol/L达到最大。外源添加苯甲酸对紫苏种子萌发与苯甲酸浓度有关（低浓度促进高浓度抑制），对紫苏幼苗的生长具有抑制作用，证实了苯甲酸对紫苏的生长存在自毒作用。

关键词: 紫苏, 苯甲酸, 种子萌发, 抗氧化酶, 化感物质

Abstract:

To investigate the effects of exogenous benzoic acid stress on seed germination, seedling growth and antioxidant enzyme activity of perilla seeds, perilla seeds petri dish filter paper culture and indoor potting test were conducted by adding benzoic acid, and six gradients were set: 0(CK), 1, 1.5, 2, 2.5 and 3 mmol/L. The effects of different concentrations of benzoic acid stress on germination percentage, germination energy, germination index, viability index, perilla seedling growth and antioxidant enzyme activities were studied. The results showed that with the increase of benzoic acid concentration, the germination rate, germination potential, germination index and viability index of perilla seeds were generally promoted by low concentration and inhibited by high concentration. The root weight, radicle length, cotyl length and plant fresh weight of perilla seedlings showed a significant downward trend, and the activities of catalase (CAT) and peroxidase (POD) in seedling leaves showed a significant decreasing trend (P<0.05), the activity of superoxide dismutase (SOD) first increased and then decreased, and reached maximum at 1.5 mmol/L. The effect of exogenous addition of benzoic acid on the germination of perilla seeds is related to the concentration of benzoic acid (low concentration promotion and high concentration inhibition), which has an inhibitory effect on the growth of perilla seedlings, confirming the self-toxicity effect of benzoic acid on the growth of perilla.

Key words: Perilla frutescens, benzoic acid, seed germination, antioxidant enzyme, allelopathic chemicals

薛彤彤, 谢萌蒙, 孙辉, 李冲伟. 外源添加苯甲酸对紫苏种子发芽和幼苗生长的影响[J]. 中国农学通报, 2024, 40(7): 72-77.

XUE Tongtong, XIE Mengmeng, SUN Hui, LI Chongwei. Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens[J]. Chinese Agricultural Science Bulletin, 2024, 40(7): 72-77.

图/表 6

参考文献 32

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浓度/(mmol/L)	发芽率/%	发芽势/%	发芽指数	活力指数
0	51.10±2.08a	17.78±3.38b	3.70±1.31bc	0.108±0.022ab
1	54.40±3.21a	37.78±1.86ab	5.43±1.09ab	0.122±0.014a
1.5	68.90±3.78a	52.22±2.16a	7.19±1.96a	0.130±0.020a
2	51.10±2.51a	34.44±1.45ab	4.98±0.29abc	0.062±0.002bc
2.5	50.00±2.00a	33.33±1.15ab	4.53±0.27bc	0.058±0.002c
3	48.90±2.51a	14.44±2.96b	3.13±0.87c	0.041±0.006c

浓度/(mmol/L)	发芽率/%	发芽势/%	发芽指数	活力指数
0	51.10±2.08a	17.78±3.38b	3.70±1.31bc	0.108±0.022ab
1	54.40±3.21a	37.78±1.86ab	5.43±1.09ab	0.122±0.014a
1.5	68.90±3.78a	52.22±2.16a	7.19±1.96a	0.130±0.020a
2	51.10±2.51a	34.44±1.45ab	4.98±0.29abc	0.062±0.002bc
2.5	50.00±2.00a	33.33±1.15ab	4.53±0.27bc	0.058±0.002c
3	48.90±2.51a	14.44±2.96b	3.13±0.87c	0.041±0.006c

浓度/(mmol/L)	胚根长/mm	胚轴长/mm	株鲜重/g
0	28.80±1.76a	15.90±1.74a	1.050±0.228abc
1	21.47±1.03b	12.10±1.01b	1.285±0.778a
1.5	19.13±1.34c	11.37±1.97b	1.025±0.393ab
2	9.27±0.50d	9.93±1.80bc	0.815±0.366bcd
2.5	7.83±0.32de	6.97±0.99c	0.550±0.160cd
3	5.8±0.46e	3.17±0.85d	0.295±0.064d

浓度/(mmol/L)	胚根长/mm	胚轴长/mm	株鲜重/g
0	28.80±1.76a	15.90±1.74a	1.050±0.228abc
1	21.47±1.03b	12.10±1.01b	1.285±0.778a
1.5	19.13±1.34c	11.37±1.97b	1.025±0.393ab
2	9.27±0.50d	9.93±1.80bc	0.815±0.366bcd
2.5	7.83±0.32de	6.97±0.99c	0.550±0.160cd
3	5.8±0.46e	3.17±0.85d	0.295±0.064d

外源添加苯甲酸对紫苏种子发芽和幼苗生长的影响

Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens

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