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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (12): 134-141.doi: 10.11924/j.issn.1000-6850.casb2023-0027

所属专题： 油料作物

• 食品·营养·检测·安全 • 上一篇    下一篇

寒地野生大豆种质资源游离氨基酸组分分析

樊超1,2(), 毕影东1(), 刘淼1, 李炜1, 刘建新1, 杨光1, 梁文卫1, 邸树峰1, 唐立郦2,3, 来永才1   

  1. 1 黑龙江省农业科学院耕作栽培研究所，哈尔滨 150001
    2 黑龙江省农业科学院博士后科研工作站，哈尔滨 150086
    3 黑龙江省农业科学院经济作物研究所，哈尔滨 150001
  • 收稿日期:2023-01-23 修回日期:2023-05-15 出版日期:2024-04-25 发布日期:2024-04-22
  • 通讯作者:
    毕影东，男，1974年出生，黑龙江哈尔滨人，研究员，博士，主要从事大豆种质资源和遗传育种。通信地址：150001 黑龙江哈尔滨松北区创新三路800号 黑龙江省农业科学院耕作栽培研究所，Tel：0451-51127898，E-mail：。
  • 作者简介:

    樊超，男，1985年出生，黑龙江哈尔滨人，助理研究员，博士，研究方向为大豆种质资源与遗传育种。通信地址：150001 黑龙江哈尔滨松北区创新三路800号 黑龙江省农业科学院耕作栽培研究所，E-mail：。

  • 基金资助:
    黑龙江省自然科学基金联合引导项目“寒地野生大豆高含硫氨基酸种质资源筛选与评价”(LH2020C099); 黑龙江省种业创新发展项目“寒地作物种质资源库改建与创新利用”(zy22001); 黑龙江省博士后基金面上项目“基于寒地野生大豆的含硫氨基酸含量QTL定位与候选基因挖掘”(LBH-Z21210)

Wild Soybean Germplasm Resources in Cold Region: Free Amino Acid Components Analysis

FAN Chao1,2(), BI Yingdong1(), LIU Miao1, LI Wei1, LIU Jianxin1, YANG Guang1, LIANG Wenwei1, DI Shufeng1, TANG Lili2,3, LAI Yongcai1   

  1. 1 Institute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin 150001
    2 Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences, Harbin 150086
    3 Institute of Cash Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150001
  • Received:2023-01-23 Revised:2023-05-15 Published:2024-04-25 Online:2024-04-22

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摘要：

旨在对采集自黑龙江省各地区的寒地野生大豆种质资源游离氨基酸组分进行深入分析。以153份寒地野生大豆为试材，利用全自动氨基酸分析仪进行了17种游离氨基酸含量的测定，并对测定结果进行了系统分析比较。结果表明：17种游离氨基酸中，甲硫氨酸变异幅度最大，变异系数为0.469，总游离氨基酸含量为2.069 mg/g，其中精氨酸含量最高，为0.894 mg/g。17种游离氨基酸含量与总游离氨基酸含量之间均为正相关，大部分游离氨基酸之间表现为正相关，仅天冬氨酸与苯丙氨酸以及丝氨酸与精氨酸之间表现为显著负相关。主成分分析提取的前5个主成分累计贡献率达85.15%，第1、2主成分贡献率分别为26.47%和20.55%。以第1、2主成分作图可知，北部地区资源和中南部地区资源之间差异较大，东部地区和西部地区资源之间差异较小。该研究明确了寒地野生大豆中17种游离氨基酸含量变异情况和组分之间的相关性，明确了不同来源地对游离氨基酸含量和组分的影响，为大豆育种应用提供了重要的基础数据。

关键词: 寒地野生大豆, 游离氨基酸, 变异系数, 相关性分析, 主成分分析

Abstract:

This study aimed to analyze the free amino acid components of wild soybean resources which were collected from various regions of Heilongjiang Province. 17 kinds of free amino acid content in 153 wild soybean resources were determined and analyzed systematically. The results indicated that the amplitude variation of methionine content was the highest among the 17 kinds of free amino acids with a variation coefficient of 0.469. The total free amino acid content was 2.069 mg/g, among which the arginine content was the highest (0.894 mg/g). Positive correlation was showed between any free amino acids and total free amino acids. There were significant positive correlations among most amino acids, while significant negative correlation could be found between aspartic acid and phenylalanine and between serine and arginine. The cumulative contribution rate of the first five principal components extracted from the principal component analysis reached 85.15%. The contribution rates of the first and second principal components were 26.47% and 20.55%, respectively. According to the first and second principal components, large discrepancies were discovered between the resources in the northern region and the central and southern regions, and small discrepancies were discovered between the resources in the eastern and western regions. In this study, the content variation and correlation among components of 17 kinds of free amino acids in wild soybean in cold region were analyzed, and the effects of different sources on the content and components of free amino acids were clarified, which provided important basic data for soybean breeding applications.

Key words: wild soybean in cold region, free amino acid, variation coefficient, correlation analysis, principal component analysis