穿心莲ApDUF247基因家族鉴定、进化与功能分析

doi:10.11924/j.issn.1000-6850.casb2025-0369

中国农学通报 ›› 2026, Vol. 42 ›› Issue (3): 99-108.doi: 10.11924/j.issn.1000-6850.casb2025-0369

穿心莲ApDUF247基因家族鉴定、进化与功能分析

张龙¹^,²(), 徐世强¹^,², 李静宇¹^,², 李旭龙¹^,², 王继华¹^,²()

¹ 广东省农业科学院作物研究所/广东省农作遗传改良重点实验室，广州 510640
² 广东省道地南药资源保护与利用工程中心，广州 510640

收稿日期:2025-05-07 修回日期:2025-10-11 出版日期:2026-02-09 发布日期:2026-02-09
通讯作者:
王继华，男，1979年出生，湖北荆门人，研究员，博士，主要从事南药资源利用研究。通信地址：510641 广东省广州市天河区五山路金颖西二街18号广东农科院作物所，E-mail：wangjihua@gdaas.cn。
作者简介:
张龙，男，2000年出生，硕士，主要从事南药资源利用。通信地址：510641 广东省广州市天河区五山路金颖西二街18号广东农科院作物所，E-mail：2539570165@qq.com。
基金资助:
广东省自然科学基金“R2R3-MYB转录因子ApMYB9调控穿心莲内酯积累的功能及分子机理”(2023A1515010426); 广东省2024年度乡村振兴战略专项资金—省级现代农业产业技术体系创新团队建设项目“以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(南药(广陈皮、化橘红等)产业技术体系)”(2024CXTD24-01); 广东省农业科学院创新基金“穿心莲ApNAC72-like转录因子调控干旱和高温胁迫的分子机制”(202305)

Identification, Evolution and Functional Analysis of ApDUF247 Gene Family in Andrographis paniculata

ZHANG Long¹^,²(), XU Shiqiang¹^,², LI Jingyu¹^,², LI Xulong¹^,², WANG Jihua¹^,²()

¹ Key Laboratory of Crop Genetic and Improvement of Guangdong Province, Crop Research Institute Guangdong Academy of Agriculture Sciences, Guangzhou 510640
² Guangdong Provincial Engineering and Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou 510640

Received:2025-05-07 Revised:2025-10-11 Published:2026-02-09 Online:2026-02-09

摘要/Abstract

摘要：

DUF247基因家族在植物生长发育和逆境响应中发挥重要作用，本研究为揭示穿心莲ApDUF247基因家族生物学功能和在胁迫中的响应机制。基于穿心莲全基因组数据，通过生物信息学方法鉴定ApDUF247基因家族成员，系统分析其理化性质、染色体定位、进化关系、基因结构、顺式作用元件分布、组织与胁迫响应表达模式、蛋白互作关系和ApDUF247与穿心莲内酯生物合成的表达相关性。结果表明，穿心莲中共有12个ApDUF247s不均等地分布于8条染色体上，其结构和功能具有多样性。穿心莲与板蓝、芝麻、拟南芥之间各有11、7、1对共线性DUF247s基因。ApDUF247s启动子区域富集激素响应、光响应和转录因子结合元件。ApDUF247s具有组织表达特异性，在不同胁迫处理下均呈现不同表达模式。ApDUF247_8与5个丝氨酸/苏氨酸蛋白激酶(STK)蛋白互作，ApDUF247_3与5个过氧化物酶体相关蛋白(Pex)和1个E3泛素化连接酶(E3s)蛋白互作，ApDUF247_6、ApDUF247_9和ApDUF247_12与穿心莲内酯生物合成通路基因具有高度表达相关性(Cor>0.8)，预测其为穿心莲内酯生物合成候选调控基因。本研究为初步解析穿心莲DUF247基因响应非生物胁迫下的生物学功能和参与穿心莲内酯生物合成调控机制奠定分析基础。

关键词: 穿心莲, DUF247, 基因表达, 基因进化, 生物学功能, 非生物胁迫, 穿心莲内酯, 调控机制, 生物信息学

Abstract:

The DUF247 gene family plays a significant role in plant growth, development, and stress responses. The purpose of this study aims to reveal the biological functions of the ApDUF247 gene family in Andrographis paniculata and its reaction mechanisms under adverse conditions. The members of the ApDUF247 gene family were identified by bioinformatics method, and their physical and chemical properties, chromosome location, evolutionary relationships, gene structure, cis-regulatory element distribution, expression patterns in different tissues and stress treatments, protein interaction and the expression correlation between ApDUF247 and andrographolide biosynthesis were analyzed. There are twelve ApDUF247 in Andrographis paniculata, which are unevenly distributed on 8 chromosomes, and their structures and functions are diverse. Andrographis paniculata has 11, 7 and 1 pairs of collinear DUF247s genes respectively with Strobilanthes cusia, Sesamum indicum and Arabidopsis thaliana. The promoter region of ApDUF247s is rich in hormone response, photoreaction and transcription factor binding elements. ApDUF247s has tissue-specific expression, which shows different expression patterns under different stress treatments. ApDUF247_8 interacts with 5 serine/threonine protein kinases, ApDUF247_3 interacts with 5 peroxisome-related proteins and 1 E3 ubiquitin lipase, and ApDUF247_6, ApDUF247_9 and ApDUF247_12 are highly correlated with the gene of the ligustrazine biosynthesis pathway (Cor>0.8), and are predicted to be candidate regulatory genes for ligustrazine biosynthesis. This study lays an analytical foundation for preliminarily understanding the biological functions of DUF247 genes in Andrographis paniculata under non-biological stress and their involvement in the regulatory mechanism of andrographolide biosynthesis.

Key words: Andrographis paniculata, DUF247, gene expression, genetics evolution, biological function, abiotic stress, andrographolide, regulatory mechanism, bioinformatics

张龙, 徐世强, 李静宇, 李旭龙, 王继华. 穿心莲ApDUF247基因家族鉴定、进化与功能分析[J]. 中国农学通报, 2026, 42(3): 99-108.

ZHANG Long, XU Shiqiang, LI Jingyu, LI Xulong, WANG Jihua. Identification, Evolution and Functional Analysis of ApDUF247 Gene Family in Andrographis paniculata[J]. Chinese Agricultural Science Bulletin, 2026, 42(3): 99-108.

图/表 8

参考文献 27

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基因ID	基因名	氨基酸长度	分子量	等电点	不稳定系数	总平均亲水性	亚细胞定位
CXN00002081-RA	ApDUF247_1	473	52700.14	8.51	48.23	-0.669	细胞质
CXN00004073-RA	ApDUF247_2	529	58971.57	5.84	47.51	-0.198	细胞质
CXN00003503-RA	ApDUF247_3	1680	186638.71	5.34	49.32	-0.115	叶绿体
CXN00012583-RA	ApDUF247_4	859	98005.69	6.31	42.15	-0.352	过氧化物酶体
CXN00000514-RA	ApDUF247_5	545	61351.73	6.01	43.98	-0.128	内质网
CXN00000675-RA	ApDUF247_6	366	41693.45	5.91	47.45	-0.297	细胞质
CXN00024588-RA	ApDUF247_7	518	59123.47	5.96	49.4	-0.309	细胞质
CXN00024587-RA	ApDUF247_8	503	57823.47	6.82	39.33	-0.298	细胞质
CXN00016149-RA	ApDUF247_9	127	14731.38	6.83	34.73	-0.276	细胞质
CXN00021843-RA	ApDUF247_10	160	18016.36	5.49	58.26	-0.642	叶绿体
CXN00014251-RA	ApDUF247_11	219	24515.95	6.38	52.31	-0.288	线粒体
CXN00018330-RA	ApDUF247_12	419	47110	7.6	41.1	-0.024	细胞质

基因ID	基因名	氨基酸长度	分子量	等电点	不稳定系数	总平均亲水性	亚细胞定位
CXN00002081-RA	ApDUF247_1	473	52700.14	8.51	48.23	-0.669	细胞质
CXN00004073-RA	ApDUF247_2	529	58971.57	5.84	47.51	-0.198	细胞质
CXN00003503-RA	ApDUF247_3	1680	186638.71	5.34	49.32	-0.115	叶绿体
CXN00012583-RA	ApDUF247_4	859	98005.69	6.31	42.15	-0.352	过氧化物酶体
CXN00000514-RA	ApDUF247_5	545	61351.73	6.01	43.98	-0.128	内质网
CXN00000675-RA	ApDUF247_6	366	41693.45	5.91	47.45	-0.297	细胞质
CXN00024588-RA	ApDUF247_7	518	59123.47	5.96	49.4	-0.309	细胞质
CXN00024587-RA	ApDUF247_8	503	57823.47	6.82	39.33	-0.298	细胞质
CXN00016149-RA	ApDUF247_9	127	14731.38	6.83	34.73	-0.276	细胞质
CXN00021843-RA	ApDUF247_10	160	18016.36	5.49	58.26	-0.642	叶绿体
CXN00014251-RA	ApDUF247_11	219	24515.95	6.38	52.31	-0.288	线粒体
CXN00018330-RA	ApDUF247_12	419	47110	7.6	41.1	-0.024	细胞质

穿心莲ApDUF247基因家族鉴定、进化与功能分析

Identification, Evolution and Functional Analysis of ApDUF247 Gene Family in Andrographis paniculata

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