不同施肥种类对金银花产量、品质及土壤微生态的影响

doi:10.11924/j.issn.1000-6850.casb2026-0018

中国农学通报 ›› 2026, Vol. 42 ›› Issue (17): 89-96.doi: 10.11924/j.issn.1000-6850.casb2026-0018

• 资源环境生态土壤气象 • 上一篇下一篇

不同施肥种类对金银花产量、品质及土壤微生态的影响

安慧玥¹(), 尚文鑫¹, 陈唱², 张明泽³, 吴舒舒⁴, 任洪建¹, 薄剑飚¹, 王良伟¹, 于李洋⁴, 韩伟⁵()

¹ 东明县农业农村局，山东菏泽 274500
² 济南市历城区政务服务中心，济南 250100
³ 寿光市文家街道农业农村服务中心，山东潍坊 262700
⁴ 菏泽市农业农村局，山东菏泽 274000
⁵ 山东省农业技术推广中心，济南 250000

收稿日期:2026-01-04 修回日期:2026-05-14 出版日期:2026-09-09 发布日期:2026-09-09
通讯作者:
韩伟，男，1977年出生，山东济南人，正高级农艺师，博士，主要从事粮食作物农业技术推广方面的研究。通信地址：250000 山东省济南市历下区解放路15号，Tel：0531-67866303，E-mail：whan01@163.com。
作者简介:
安慧玥，女，2000年出生，山东威海人，助理农艺师，硕士，主要从事金银花与农业技术推广研究。通信地址：274500 山东省菏泽市东明县曙光路27号东明县农业农村局农业技术股，E-mail：842740426@qq.com。
基金资助:
国家重点研发计划“寡糖类复合新材料在山东典型农作物上的应用试验及示范推广”(2019YFE0197100-3)

Effects of Different Fertilizer Types on Yield and Quality and Soil Microecology of Lonicera japonica

AN Huiyue¹(), SHANG Wenxin¹, CHEN Chang², ZHANG Mingze³, WU Shushu⁴, REN Hongjian¹, BO Jianbiao¹, WANG Liangwei¹, YU Liyang⁴, HAN Wei⁵()

¹ Dongming Agriculture and Rural Bureau, Heze, Shandong 274500
² Licheng District Government Service Center, Jinan 250100
³ Wenjia Street Agricultural and Rural Service Center, Shouguang, Weifang, Shandong 262700
⁴ Heze Agriculture and Rural Bureau, Heze, Shandong 274000
⁵ Shandong Agricultural Technology Promotion Center, Jinan 250000

Received:2026-01-04 Revised:2026-05-14 Published:2026-09-09 Online:2026-09-09

摘要/Abstract

摘要：

本研究以当地种植的‘四季’金银花(Lonicera japonica)为试验材料，探究不同施肥处理对金银花产量、品质及根际土壤微生态的影响，以期为金银花的科学施肥管理提供科学依据。试验采用单因素设计，初步确定最佳施肥处理后，设置增施有机肥、增施生物菌肥和仅施基肥(CK)对照3个处理组，通过16S rRNA基因扩增子测序技术，并对根际土壤细菌群落进行分析。同时，测定了金银花花蕾的鲜重、干重、长度、腺毛数量、绿原酸含量和产量，全面评估了不同肥料处理对金银花产量和品质的效应，并结合根际土壤细菌群落的变化，分析探讨其土壤微生态调控机制。结果表明，增施有机肥显著提升了金银花的各项品质指标：花蕾长度较对照组平均增加1.24 mm，腺毛数量和绿原酸含量比对照分别提高4.13%、17.99%。增施有机肥处理的金银花产量分别比增施生物菌肥和对照提高了5.61%、9.51%。不同的施肥处理均能改变了金银花根际土壤细菌群落的组成结构。综上，不同的施肥策略对当地金银花的产量和生长指标均产生显著影响，其中增施有机肥表现出最优越的效果，为当地金银花的科学施肥管理策略提供了重要参考。

关键词: 金银花, 施肥, 绿原酸, 产量, 土壤细菌

Abstract:

This study used locally cultivated ‘Siji’ Lonicera japonica as the experimental material to investigate the effects of different fertilization treatments on the yield, quality, and rhizosphere soil microecology of Lonicera japonica, with the aim of providing a scientific basis for its fertilization management. A single-factor experimental design was adopted. After the optimal fertilization treatment was preliminarily determined, three treatments were established, including supplemental application of organic fertilizer, supplemental application of microbial fertilizer, and basal fertilizer only as the control (CK). The bacterial communities in the rhizosphere soil were analyzed by sequencing technology. The effects of different fertilization treatments on the yield and quality of Lonicera japonica were evaluated by measuring the fresh weight, dry weight, length, number of glandular trichomes, chlorogenic acid content, and yield of flower buds. In addition, the soil microecological effects were explored in combination with the analysis of rhizosphere soil bacterial communities. The results showed that supplemental application of organic fertilizer increased flower bud length, the number of glandular trichomes, yield, and chlorogenic acid content of Lonicera japonica. Compared with the control, flower bud length increased by 1.24 mm, while the number of glandular trichomes and chlorogenic acid content increased by 4.13% and 17.99%, respectively. Following supplemental application of organic fertilizer, the yield of Lonicera japonica increased by 5.61% and 9.51% compared with supplemental microbial fertilizer application and the control, respectively. Meanwhile, fertilization treatments altered the composition of bacterial communities in the rhizosphere soil of Lonicera japonica. In conclusion, different fertilization treatments had varying effects on the yield and growth indicators of locally cultivated Lonicera japonica, among which supplemental application of organic fertilizer showed the best performance. These findings provide an important reference for fertilization management in local Lonicera japonica cultivation.

Key words: Lonicera japonica, fertilization, chlorogenic acid, yield, soil bacteria

中图分类号:

S567.79，S143

安慧玥, 尚文鑫, 陈唱, 张明泽, 吴舒舒, 任洪建, 薄剑飚, 王良伟, 于李洋, 韩伟. 不同施肥种类对金银花产量、品质及土壤微生态的影响[J]. 中国农学通报, 2026, 42(17): 89-96.

AN Huiyue, SHANG Wenxin, CHEN Chang, ZHANG Mingze, WU Shushu, REN Hongjian, BO Jianbiao, WANG Liangwei, YU Liyang, HAN Wei. Effects of Different Fertilizer Types on Yield and Quality and Soil Microecology of Lonicera japonica[J]. Chinese Agricultural Science Bulletin, 2026, 42(17): 89-96.

图/表 8

类型	区域	引物序列
细菌	16S V3-V4	CCTAYGGGRBGCASCAG,GGACTACNNGGGTATCTAAT

表1. 各区域引物序列

图1. 施肥处理对不同时间采集的金银花形态的影响 a：5月；b：6月；c：7月；d：8月。比例尺：1 cm

图2. 不同肥料对不同取花时期金银花鲜重和干重的影响

图3. 不同肥料对不同取花时期金银花花蕾长度的影响

图4. 不同肥料对不同取花时期金银花腺毛数量的影响

图5. 不同肥料对不同取花时期金银花绿原酸含量的影响

图6. 不同肥料处理对金银花产量的影响

图7. 不同处理对微生物群落组成的影响 M1、M2、M3分别为金银花花蕾期对照、有机肥、生物菌肥3个处理；M4、M5、M6分别为盛花期对照、有机肥、生物菌肥3个处理。 A：对照、有机肥、生物菌肥组土壤微生物门水平群落组成；B：对照、有机肥、生物菌肥组土壤微生物纲水平群落组成

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不同施肥种类对金银花产量、品质及土壤微生态的影响

Effects of Different Fertilizer Types on Yield and Quality and Soil Microecology of Lonicera japonica

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