果树病毒核酸检测技术研究进展

摘要/Abstract

摘要： 病毒的核酸检测技术发展非常迅速，主要有PCR技术、分子杂交技术、dsRNA技术、NASBA技术和基因芯片技术。综述了这些技术的原理、特点及其在果树病毒检测中的应用情况。基于PCR技术的检测方法是目前果树病毒检测的主要方法，许多果树病毒的PCR检测体系已经建立。NASBA技术也已开始用于果树病毒的检测。

关键词: 退耕地, 退耕地, 前茬作物, 人工草地, 组合, 三峡库区

Abstract: Detection techniques based on nucleic acid for plant viruses, including of PCR, molecular hybridization, dsRNA, NASBA and DNA chip, were developed rapidly. The principle, characteristics and applications of these techniques in detection of fruit tree viruses are reviewed. At present, the detection methods based on PCR are the main methods for fruit tree viruses, and many fruit tree viruses have been detected by PCR. NASBA is applied to the detection of fruit tree virus recently.

中图分类号:

S668

杨洪一,李丽丽,张志宏. 果树病毒核酸检测技术研究进展[J]. 中国农学通报, 2007, 23(2): 350-350.

Yang Hongyi , Li Lili , Zhang Zhihong. Advances of Detection Techniques for Fruit Tree Viruses Based on Nucleic Acids[J]. Chinese Agricultural Science Bulletin, 2007, 23(2): 350-350.

参考文献

[1] Converse R H. Virus diseases of small fruits. USA: US Dep. Agric, 1987: 16-29.
[2] Schoen C D, Leone G. Towards molecular detection methods for aphid-borne strawberry viruses. Acta Horticulturae, 1995, 385: 55-63.
[3] Stenger D C, Mullin R H, Morris T J. Isolation, molecular cloning, and detection of Strawberry vein banding virus DNA. Phytopathology, 1988, 78: 154-159.
[4] Petrik K, Benes V, Mraz I, et al. Strawberry vein banding virus-definitive member of the Genus Caulimovirus. Virus Genes, 1998, 16: 1-3.
[5] Jelkmann W, Kunze L V, Ethen H J. cDNA cloning of dsRNA and evidence for relationship of the virus-like agents associated with apple stem potting and pear vine yellow. Acta Horticulture, 1992, 309: 55-62.
[6] Jelkmann W. Cherry virus A cDNA cloning of dsRNA, nucleotide sequence analysis and serology reveal a plant capillovirus in sweet cherry. Journal of General Virology, 1995, 76: 2015-2024.
[7] Thompson J R, Leone G, Lindner J L, et a1. Characterization and complete nucleotide sequence of Strawberry mottle virus：a tentative member of a new family of bipartite plant picorna-like viruses. Journal of General Virology, 2002, 83: 229-239.
[8] Karasev, Boyker. Complete sequence of Citrus tristeza virus RNA genome. Virology, 1995, 208(2): 511-520.
[9] Posthuma K I, Adams A N, Hong Y, et al. Detection of Strawberry crinkle virus in plants and aphids by RT-PCR using conserved L gene sequences. Plant Pathology, 2002, 51: 266-274.
[10] Thompson J R, Wetzel S, Klerks M M, et al. Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. Journal of Virological Methods, 2003, 111: 85-93.
[11] Hammond R W, Crosslin J M, Pasini R, et al. Differentiation of closely related but biologically distinct cherry isolates of prunus necrotic ringspot virus by polymerase chain reaction. Journal of Virological Methods, 1999, 80: 203-212.
[12] Dovas C I, Katis N I. A spot multiplex nested RT-PCR for the simultaneous and generic detection of viruses involved in the aetiology of grapevine leafroll and rugose wood of grapevine. Journal of Virological Methods, 2003, 109: 217-226.
[13] Candresse T, Lanneau M, Revers F, et al. An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the Apple chlorotic leafspot virus. Acta Horticulturae, 1995, 386: 136-147.
[14] Regner F, Stadlbauer A, Eisenheld C. Broad range detection of different Strawberry latent ringspot virus isolate by immunocapture PCR. Mitteilungen Klosterneuburg., Rebe-und-Wein, Obstbau-und- Fruchteverwertung. 2000, 50(6):183-191.
[15] Sefc K M, Leonhardt W, Steinkellner H. Partial sequence identification of grapevine-leafroll-associated virus-1 and development of a highly sensitive IC-RT-PCR detection method. Journal of Virological Methods, 2000, 86: 101-106.
[16] Kaden-Kreuziger D, Lamprecht S, Martin R R, et al. Immunocapture polymerase chain reaction assay and ELISA for the detection of Strawberry mild yellow edge associated potexvirus. Acta Horticulturae, 1998, 385: 33-40.
[17] Jelkmann W, Keim-Konrad R. An immuno-capture polymerase chain reaction and plate-trapped ELISA for the detection of Apple stem pitting virus. Journal of Phytopathol, 1997, 145: 499-504.
[18] Harper G, Dahal G, Thottappilly G, et al. Detection of episomal Banana streak badnavirus by IC-PCR. Journal of Virological Methods, 1999, 79: 1-8.
[19] Olmos A, Dasi M A, Candresse T, et al. Print-capture PCR: a single and highly sensitive method for the detection of Plum pox virus(PPV) in plant tissues. Nucleic Acids Research, 1996, 24(11):2192-2193.
[20] Candresse T, Kofalvi S A, Lanneau M, et al. A PCR-ELISA procedure for the simultaneous detection and identification of Prunus necrotic ringspot and Apple mosaic ilarviruses. Acta Horticulturae, 1998, 472: 219-225.
[21] Delanoy M, Salmon M, Kummert J. et al. Development of real-time PCR for the rapid detection of episomal Banana streak virus(BSV). Plant Disease, 2003, 87: 33-38.
[22] Salmon M, vendrame M, Kummert J, et al. Detection of Apple cholorotic leaf spot virus using a 5' nuclease assay with a fluorescent 3' minor groove binder-DNA probe. Journal of Virological Methods, 2002, 104: 99-106.
[23] Salmon M, Vendrame M, Kummert J, et al. Rapid and homogeous detection of Apple stem pitting virus by RT-PCR and a fluorogenic 3'minor groove binder-DNA probe. European Journal of Plant Pathology, 2002, 108: 755-762.
[24] James D W, Jelkmann, Upton C. Single primer pair designs that facilitate simultaneous detection and differentiation of Peach mosaic virus and Cherry mottle leaf virus. Journal of Virological Methods, 1999, 83: 103-110.
[25] Nemichov L, Hadidi A, Candress T, et al. Sensitive detection of Apple chlorotic leaf spot virus from infected apple or peach tissue using RT-PCR, IC-RT-PCR, or multiplex IC-RT-PCR. Acta Horticulturae, 1995, 386: 51-62.
[26] Menzel W, Jelkmann W, Maiss E. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. Journal of virological methods, 2002, 99: 81-92.
[27] Nassuth A, Pollari E, Helmeczy K, et al. Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts. Journal of Virological Methods, 2000, 90: 37-49.
[28] Saade M, Aparicio F, Sanche-navarro JA, et al. Simultaneous detection of the three Illaviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction. Phytopathology, 2000, 90: 1330-1336.
[29] Ito T, Ieki H, Ozaki K. Simultaneous detection of six citrus viroids and apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction. Journal of Virological Methods, 2002, 106: 235-239.
[30] Webster G C, Wylie S J, Jones M G K. Diagnosis of plant viral pathogens. Current Science, 2004, 86:1604-1607.
[31] Mraz I, Petrzik K, Chvalova D, et al. Experiences with testing of Strawberry vein banding virus in strawberries by PCR and dot blot hybridization. Journal of plant diseases and protection, 1999, 3: 1-6.
[32] Mas P, Sanchez-Navarro J A, Sanchez-Pina M A, et al. Chemiluminescent and colorigenic detection of Cherry leaf roll virus with digoxigenin-labeled RNA probes. Journal of Virological Methods, 1993, 45(1): 93-102.
[33] Rott M E, Jelkmann W. Characterization and detection of several filamentous viruses of cherry: adaptation of an alternative cloning method (DOP-PCR),and modification of an RNA extraction protocol. European Journal of Plant Pathology, 2001, 107: 411-420.
[34] Zhang Y P, Jerry K, Uyemoto, et al . Nucleotide sequence and RT-PCR detection of a virus associated with grapevine rupestris stem-pitting disease. Phytopathology, 1998, 88: 1231-1237.
[35] Guatelli J, Whitfield K, Kwoh D, et al. Isothermal in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of the National Academy of Sciences of the United States of America, 1990, 87: 1874-1878.
[36] Lair S V, Mirkov T E, Dodds J A, et al. A single temperature amplification technique applied to the detection of citrus tristeza viral RNA in plant nuclei acid extracts. Journal of Virological Methods, 1994, 47: 141-151.
[37] Vaskova D, Spak J, Taborsky V, et al. The use of PCR and NASBA for detection of Strawberry Vein Banding Virus (SVBV). Plant Protection Science, 2002, 38: 24-27.
[38] Klerks M M, Lindner J L, Vaskova D. Detection and tentative grouping of Strawberry crinkle virus isolates. European Journal of Plant Pathology, 2004, 110: 45-52.
[39] Klerks M M, Heuvel J F, Van den Heuvel J F, et al. Detection of nematode-transmitted Nepoviruses by the novel, one-tube amplidet RNA assay. Acta Horticulturae, 2001, 550: 53-58.
[40] Nakahara K, Hataya T, Uyeda I. Inosine 5’-triphosphate can dramatically increase the yield of NASBA products targeting GC-rich and intramolecular base-paired viroid RNA. Nucleic Acids Research, 1998, 26: 1854-1855.
[41] Klerks M M, Leone M, Verbeek, J F, et al. Development of a multiplex AmpliDet RNA for the simultaneous detection of Potato leafroll virus and Potato virus Y in potato tubers. Journal of Virological Methods, 2001, 93: 115-125.
[42] Szemes M, Klerks M M, Van den Heuvel J F, et al. Development of a multiplex AmpliDet RNA assay for simultaneous detection and typing of Potato virus Y isolates. Journal of Virological Methods, 2002, 100: 83-96.
[43] Boonham N, Walsh K, Smith P, et al. Detection of potato viruses using microarray technology: towards a generic method for plant viral disease diagnosis. Journal of Virological Methods, 2003, 108: 181-187.