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中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 1-1.

• 林学 园艺 园林 • 上一篇    下一篇

台湾桤木花粉生活力的测定方法初探

王旭军,吴际友,程 勇,廖德志   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Study on Determination Methods of Alnus formosana Pollen Viability

Wang Xujun, Wu Jiyou , Cheng Yong, Liao Dezhi   

  • Online:2007-09-05 Published:2007-09-05

摘要: 【研究目的】探讨台湾桤木花粉萌发的适宜培养基组成、培养温度及台湾桤木花粉生活力的快速测定方法。【方法】以台湾桤木新鲜花粉为试材,采用花粉离体培养萌芽法及TTC染色法、I2-KI染色法、过氧化物酶沉淀法等方法进行研究。【结果】台湾桤木花粉离体培养的适宜培养液为:10%蔗糖+100mg/L硼酸;最适培养温度为25℃。过氧化物酶沉淀法是快速测定台湾桤木花粉生活力的适宜方法。【结论】蔗糖和硼是台湾桤木花粉萌发的必要条件

关键词: 果树, 果树, 叶幕, 微区光环境, 叶幕微气候, 太阳辐射

Abstract: 【OBJECTIVE】In order to study the suitable media components, temperture of Alnus formosana pollen, and the fast detetminination method of its pollen viability.【METHOD】With Fresh pollen of Alnus formosana as the experimental material, In vitro culture and 3 staining tests(TTC staining, I2-KI staining and peroxidase settling process) were conducted to determine the pollen viability.【RESULTS】The optimum culture solution was sucrose 10g/L+H3BO3 10mg/L.Peroxidase settling process was the optimum staining for the fast determination of Alnus formosana pollen viability. 【CONCLUSION】Sucrose and H3BO3 were essential for pollen germination of Alnus formosana.

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