棉花EST-SSRs在香蕉中的通用性

摘要/Abstract

摘要： 香蕉SSR分子标记开发和应用有限,通过对棉花SSR分子标记在香蕉中的通用性研究.用1343对棉花EST-SSR引物对贡蕉(AA)和野蕉(BB)两个材料进行转移扩增,得到226对有效扩增的引物.进一步用该226对引物对20个香蕉品种和4个野蕉材料扩增,得到了157对多态性引物.157对多态性引物共扩增到1188个条带,平均7.6个;引物的多态信息含量范围在0.58～0.91之间.依据获得的SSR数据,应用非加权类平均聚类方法,在相似系数0.60水平上,将24个品种分为两大类群,类群Ⅰ是以A基因组为主的品种群,类群Ⅱ是以B基因组和A基因组与B基因组杂交的品种群,证明了棉花EST-SSR分子标记在香蕉中具有通用性.

关键词: 古槐树, 古槐树, 保护, 复壮, 延缓衰老

中图分类号:

S668

郑丽珊　石玉真　王静毅　黄秉智　冀小蕊　张保才　袁有禄　武耀. 棉花EST-SSRs在香蕉中的通用性[J]. 中国农学通报, 2008, 24(1): 33-37.

Zheng Lishan　 Shi Yuzhen　 Wang Jingyi　 Huang Bingzhi　 Ji Xiaorui　 Zhang Baocai　 Yuan Youlu　 Wu Yaoting. Transferability of Cotton EST-SSRs Markers to Musa[J]. Chinese Agricultural Science Bulletin, 2008, 24(1): 33-37.

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