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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 93-96.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

辣椒推定WRKY基因cDNA的生物信息学分析

王育娜,,贺 俐,曹 蕾,赖 燕,牟少亮,何水林   

  • 收稿日期:2008-09-24 修回日期:1900-01-01 出版日期:2008-11-05 发布日期:2008-11-05

Bioinformation Analyze of a Pepper Putative WRKY gene

Wang Yuna,, He Li, Cao Lei, Lai Yan, Mou Shaoliang, He Shuilin   

  • Received:2008-09-24 Revised:1900-01-01 Online:2008-11-05 Published:2008-11-05

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摘要： 本研究在利用同源克隆法分离到一个推定的辣椒WRKY 转录因子的基础上，采用多种生物信息学方法对该序列进行特征分析和功能预测, 以获得该基因及其编码蛋白更多的功能提示。方法: 应用B1ast、ORF Finder、Wolf Psort、DNAMAN等软件或数据库, 进行序列的相似性比较, 开放读码框预测、保守域和编码蛋白质的功能等分析。结果: 我们得到的WRKY序列是该基因家族中的一个全长cDNA序列, 该基因编码的蛋白具有WRKY保守结构域和锌指结构特征, 亚细胞定位预测结果显示该蛋白定位于细胞核。结论: 辣椒WRKY新基因是一新成员, 进化上保守有保守性, 可能在在调控植物的生长发育以及干旱、高温、高盐等逆境应答过程中起重要调节作用。因此, 这一辣椒WRKY新基因有进一步研究的价值。

关键词: Bt棉, Bt棉, 钾肥, 产量, 效应

Abstract: Abstract: A pepper cDNA library was screened to look for the WRKY homologous molecules. Then a novel cDNA was found during the screen and cloned. Bioinformatics analysis was performed in order to study the possible function of this cDNA sequence. Various bioinformatics methods such as blastn, ORF Finder, Wolf Psort and DNAMAN were used to analyze and predict the feature and possible functions of the novel cDNA sequence. The result shows Putative WRKY was the full-length of a novel gene and there was a conserved WRKY domain predicted by NCBI, the homologous analysis shows WRKY was highly identity with other plants and predicted to locate in nuclei. It may function in plant development and play a role during resistance to various environmental. Therefore, the functions of the novel gene are valuable for further investigations.

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