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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 270-273.

所属专题： 生物技术

• 水产 渔业 • 上一篇    下一篇

缢蛏致病菌气单胞菌的分子生物学鉴定

王兴强,曹 梅,阎斌伦,等   

  • 收稿日期:2008-10-24 修回日期:2008-12-19 出版日期:2009-02-20 发布日期:2009-02-20

Identification of Pathogenic Aeromonas from Sinvnovacula constricta by Molecular Biology

  • Received:2008-10-24 Revised:2008-12-19 Online:2009-02-20 Published:2009-02-20

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摘要： 从患病缢蛏（Sinvnovacula constricta）中分离到一株病原菌，暂命名为12#菌。对该菌株的16S rDNA的全序列进行PCR扩增并测序，然后用NCBIBLAST对测序结果进行比对。16S rDNA序列分析表明：12#菌与多株气单胞菌的16S rDNA的同源性均在95%以上。在细菌系统分类学中应归属于气单胞菌属。从构建的系统发育树中可以看出：12#菌与点状产气单胞菌（Aeromonas punctata）共同构成一个分支，且该菌株与点状产气单胞菌（Aeromonas punctata）的16S rDNA同源性达到99%以上，由此可以初步认为该菌为点状产气单胞菌（Aeromonas punctata）。

关键词: MAPK12基因, MAPK12基因, 猪, ORF, 序列分析

Abstract: One pathogenic strain originally isolated from Sinvnovacula constricta was named 12#. 16S rDNA of strain 12# was amplified by PCR and the full-length of 16S rDNA sequence was determined. Using the NCBI BLAST database, this sequence was compared. The 16S rDNA sequence analysis indicated that strain 12# shared above 95% homology with some Aeromonas punctata. In the hylogenetic framework of bacterial classification, the strain 12# belongs to Aeromonas punctata. In phylogenetic tree, it was concluded that stain 12# and Aeromonas punctata sequences constituted a branch and this strain shared above 99% 16S rDNA sequence homology with Aeromonas punctata. So, we assure that strain 12# is Aeromonas punctata. Keywords: Sinvnovacula constricta，Aeromonas punctata，16S rDNA，Phylogenetic analysis，Identification.

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