不同培养代数鹿茸生长中心细胞对IGF1刺激 的反应

摘要/Abstract

摘要： 【研究目的】探讨胰岛素样生长因子（IGF1）对生长60 d的梅花鹿鹿茸体外培养不同代数鹿茸生长中心细胞的影响。【方法】分离、培养生长60d的梅花鹿鹿茸生长中心细胞，将培养第2 代、5 代、8 代细胞经含有不同浓度的IGF1（0，1，3 和10 nM）作用，在培养24 h后用3H-胸腺嘧啶核苷掺入测定法检测每分钟衰变数（DPM）值。【结果】全部IGF1处理组都显著高于对照组（p<0.01）。不同浓度IGFⅠ处理组的平均值和最高值都是离体培养2 代的细胞的DPM最高（分别为31918和39818 DPM/mg蛋白），培养5 代的细胞（分别为5455和6815 DPM/mg蛋白）已大大地下降；到了第 8代的（分别为4030和4838 DPM/mg蛋白）又有进一步的下降。【结论】IGF1能够促进鹿茸生长中心细胞分裂增殖，生长60 d鹿茸的生长中心细胞在离体培养，不同培养时期的鹿茸生长中心细胞对IGFⅠ刺激的敏感度不同。

关键词: 土地整理, 土地整理, 景观生态, 生态平衡

Abstract: 【Objective】To investigate the effects of insulin-like growth factor 1 (IGF1)on the proliferation of antler organic center cells from various passages in vitro at 60 days growing period of antler. 【Method】Antler organic center cells were isolated from sika deer antler of growing 60 day and cultured. The second, fifth and eighth population cells were incubated with 0, 1, 3, and 10 nM IGF1. Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the disintegration per minute ( DPM) value.【Results】The proliferation of cells from growing 60 day’s antler in the experimental groupwas significantly higher than that of the control group (p<0.01). The average and maximal DPM value of the proliferation of cells in the experimental group was the highest in the second population cells (31918 and 39818 DPM/mg respectively), decreased in the fifth population cells materially (5455 and 6815 DPM/mg), and decreased in the eighth population cells again (4030 and 4838 DPM/mg). 【Conclusion】The results show that IGF1 can enhance the proliferation of antler organic center cells cultured in vitro. The sensitivity of IGF1 on the antler organic center cells from various passages at 60 days growing period of antler in vitro.

梅妹,邓旭明,岳占碰,赵丽红,李春义,冯海华. 不同培养代数鹿茸生长中心细胞对IGF1刺激的反应[J]. 中国农学通报, 2008, 24(4): 1-3.

Mei Mei, Deng Xuming, Yue Zhanpeng, Zhao Lihong, Li Chunyi, Feng Haihua. Effcets of Insulin-Like Growth Factor 1 on the Proliferation at Different Generation of Antler Organic Center Cells[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 1-3.

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