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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (9): 287-292.

所属专题： 生物技术； 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

两种真姬菇摇瓶发酵产生菌丝体及
胞外多糖研究

刘成荣,邓百万   

  • 收稿日期:2008-04-01 修回日期:2008-06-22 出版日期:2008-09-05 发布日期:2008-09-05

Study on the Mycelia and Extra Cellular Polysaccharide of
Two speice of Hypsizigus marmoreus by Rotation Fermentation

Liu Chengrong, Deng Baiwan   

  • Received:2008-04-01 Revised:2008-06-22 Online:2008-09-05 Published:2008-09-05

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783

摘要： 采用摇瓶培养法对2种真姬菇的发酵工艺进行研究，结果表明：真姬菇Ⅰ、Ⅱ发酵生产菌丝体及胞外多糖的碳源是葡萄糖，氮源是酵母膏；液体发酵培养基是葡萄糖3%、酵母膏 0.4%、vitB1 10mg/100ml、KH2PO4 0.1%、MgSO4 0.1%，pH值为6.0；液体发酵的优化条件是初始pH 6.0~7.0，振荡速度110~130r/min，培养温度25~28℃，装液量150ml/250ml。2种真姬菇菌丝体及胞外多糖产量较高，真姬菇Ⅰ菌丝体干重、胞外多糖产量分别是 3.92、8.83 mg /ml；真姬菇ⅠⅠ菌丝体干重、胞外多糖产量分别是3.25、7.69 mg /ml，即真姬菇Ⅰ摇瓶发酵菌丝体干重、胞外多糖产量高于真姬菇Ⅱ。

关键词: 肯定列表制度, 肯定列表制度, 食品, 农产品, 出口, 影响, 应对

Abstract: According to the research of the fermentation technique of two speice of Hypsizigus marmoreus by rotation culture .The result is: glucose is the carbon source of mycelium and extra cellular polysaccharide of Hypsizigus marmoreusⅠ and Hypsizigus marmoreusⅡ in fermentation, yeast extractea is the nitrogen source; liquid fermentation medium consist of 3% glucose, 0.4% yeast extractea, vitB1 10mg/100ml、KH2PO4 0.1%、MgSO4 0.1%，and PH value is 6.0; optimum condition for liquid fermentation includes: pH 6.0~7.0，bed rotation speed 110~130r/min，temperature 25~28℃, volum 150ml/250ml at the beginning. mycelium and extra cellular polysaccharide of two speice of Hypsizigus marmoreus have high production and they are: 3.92mg/ml for the dry weight of mycelium and 8.83 mg /ml for extra cellular polysaccharide of Hypsizigus marmoreusⅠ; while 3.25mg/ml for the dry weight of mycelium and 7.69 mg /ml for extra cellular polysaccharide of Hypsizigus marmoreus Ⅱ, The production of the dry weight of my mycelium and extra cellular polysaccharide of Hypsizigus marmoreus Ⅰin rotation fermentation is more than Hypsizigus marmoreusⅡ,s.

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