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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (14): 63-66.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

紫菜薹总DNA的快速提取与SSR分子标记鉴定

白占兵,,丁茁荑,,李雪峰,,等   

  • 收稿日期:2009-02-09 修回日期:2009-03-30 出版日期:2009-07-20 发布日期:2009-07-20

Rapid Extraction Total DNA from Purple Tsai-tai and Characterization with SSR Molcular Marker

Bai Zhanbing,, Ding Zhuoyi,, Li Xuefeng,,等   

  • Received:2009-02-09 Revised:2009-03-30 Online:2009-07-20 Published:2009-07-20

PDF (PC)

961

摘要： 为充分利用原产于我国的紫菜薹资源，能够快速提取它的总DNA是利用分子生物学研究紫菜薹的基础。本方法采用简化的CTAB法，可快速大批量的提取紫菜薹的DNA ，提取速度和用试剂盒提取相当，提取量远大于试剂盒，本方法也适应对其它作物。

关键词: 梨, 梨, 贮藏, 褐变, 酚类物质, 酶活性

Abstract: To take full advantage of originating in China's Purple Tsai-tai resources. It can quickly extract the total DNA is the use of molecular biology research foundation. This method is a simplified CTAB method, can rapid extraction of large quantities of Purple Tsai-tai DNA, Extraction rate equivalent to the speed with extraction kit. Extraction amount is much larger than the kit, which can also adapt to other crops.