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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 61-64.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

钼酸铵法测定水稻过氧化氢酶(CAT)活性

彭建,王丹英,徐春梅,陈丽萍,邓飞,章秀福   

  • 收稿日期:2009-02-27 修回日期:2009-06-06 出版日期:2009-08-20 发布日期:2009-08-20

Ammonium Molybdate Method for Detecting the Activities of Rice Catalase

PENG Jian , , , , ,   

  • Received:2009-02-27 Revised:2009-06-06 Online:2009-08-20 Published:2009-08-20

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1664

摘要： 摘 要：钼酸铵法测定植物CAT活性，具有准确、方便、高效的特点。以内两优6号、秀水09的剑叶为材料，通过与紫外吸收法准确度和精确度的比较，来验证该方法的可靠性。①两种方法结果均值接近，没有显著性差异。②紫外吸收法的变异系数分别是0.78%和0.58%，钼酸铵法的变异系数分别是0.35%和0.42％

关键词: 鸡腿菇, 鸡腿菇, 玉米秸秆, 菌丝, 子实体

Abstract: Abstract:To detect the activities of rice catalase ,ammonium molydate method is accurate, convenient and efficient .The reliability of the method was verified by using flag leaf of Neiliangyou 6 and Xiushui 09 and comparing the accuracy and the precision with ultraviolet absorption method.The first,the average of two method have no significent difference .The second, the Coefficient of Variation of ultraviolet absorption method respectively is 0.78% and 0.58%,and the Coefficient of Variation of ammonium molydate method respectively is 0.35% and 0.42％.