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中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 65-68.

所属专题: 水稻

• 生物技术科学 • 上一篇    下一篇

水稻高效花药培养技术体系的构建

王伍梅,台德卫,张效忠,钱征,林翠香   

  • 收稿日期:2009-04-27 修回日期:2009-05-13 出版日期:2009-08-20 发布日期:2009-08-20

Construction of highly efficient technology system of anther culture on rice

Wang Wumei, Tai Dewei, Zhang Xiaozhong, Qian Zhen, Lin Cuixiang   

  • Received:2009-04-27 Revised:2009-05-13 Online:2009-08-20 Published:2009-08-20

摘要: 为提高水稻花药培养效果,产生大量的花粉植株,以多年的试验并结合多方面的研究结果,论述了水稻花药培养各个环节中的关键技术,创建了通常情况下的一套水稻高效花药培养技术体系。本系统着重于在利用基因型的选择和基本配养基的交叉使用以提高花药愈伤组织诱导率的的前提下,在其它的每个环节,尤其是壮苗和移植管理技术方面进行了优化改进,以提高绿苗分化率和组培苗移植后的成活率。

关键词: 缓释, 缓释, 氮, 表观利用率, 肥效

Abstract: In order to enhance the efficiency of anther culture of rice and culture a large of green plant of pollen, some critical technologies of anther culture was discussed through the effects of our trails and other studies of anther culture on rice,and then a highly efficient technology system of anther culture on rice for common condition was Constructed . This system suggested that, in the condition of enhancing the Callus inducting rate in the way of the selection of gene types and alternate using of induction media, improve on other techniques specially the techniques of plant conducted and managements of green plantlet planting and then improving the plant regeneration rate and living rate of green plantlet planting.