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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 71-73.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

RAPD分子标记在苹果梨的分类地位鉴定中的应用

马艳芝1,2,张玉星1   

  • 收稿日期:2009-04-21 修回日期:2009-05-11 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    梨属种质资源分子遗传连锁图谱构建与遗传多样性分析

Application RAPD on Identification of systematic status of Pingguoli pear

MA Yan-Zhi   

  • Received:2009-04-21 Revised:2009-05-11 Online:2009-09-20 Published:2009-09-20

PDF (PC)

622

摘要：

【研究目的】苹果梨是梨属中优良的种质资源，但关于其分类地位一直存在争议，为苹果梨的分类地位提供DNA水平上的依据。【方法】本研究利用RAPD分子标记技术，通过聚类分析和遗传相似系数分析，对其进行分类研究。【结果】结果表明：当λ=12时苹果梨均首先与其各自杂交品种聚在一起后，再与白梨系统的其它类型聚在一起；苹果梨与秋子梨系统、白梨系统、砂梨系统、西洋梨系统的代表品种间的平均欧式距离分别是6.099、5.022、5.713、6.703。【结论】认为将苹果梨归为白梨系统较为适宜。

关键词: 水稻, 水稻, 超高产, 栽培

Abstract:

【OBJECTIVE】Pingguoli pear were excellent germplasm resource with cold resistance, but the ownerships of it was not find out clearly. The objective was to find out the ownership of Pingguoli pear. 【METHOD】 Using RAPD markers, delving and clearing the genetic relationships of partly Pyrus germplasm was studied, by the existent demurral and the dendrogram. 【RESULTS】By the existent demurral on systematic status of Pingguoli pear, the general Euclidean distances between P. Bretschneideri Rehd., P.pyrifolia Nakai, P. ussuriensis Maxim, P. communis L. and Pingguoli pear were 5.022, 5.713, 6.099, and 6.703 respectively. From the dendrogram, Pingguoli pear was clustered with P. Bretschneideri Rehd. 【CONCLUSION】That was to say Pingguoli pear should be most similar to White-pear system.