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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (21): 22-25.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

利用荧光定量PCR方法研究猪繁殖与呼吸综合征病毒在Marc-145细胞的增殖规律

胡 慧1,2,高晓平2,孟韩冰1,王亚宾2,陈丽颖1,张红英1,辛银川2,崔保安2   

  • 收稿日期:2009-04-27 修回日期:2009-07-26 出版日期:2009-11-05 发布日期:2009-11-05
  • 基金资助:

    福建省科技重大专项;河南省杰出人才创新基金项目

Study on the replication of Porcine Reproductive and Respiratory Syndrome Virus in Marc-145 Cells using Fluorescence Quantitative PCR

  • Received:2009-04-27 Revised:2009-07-26 Online:2009-11-05 Published:2009-11-05

PDF (PC)

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摘要：

摘要:猪繁殖与呼吸综合征病毒可以在原代猪肺泡巨噬细胞(PAM)、CL2621细胞系及Marc-145细胞系上生长繁殖，但对于其增殖规律不甚了解。试验利用实时荧光定量PCR技术，研究猪繁殖与呼吸综合征病毒在Marc-145细胞中的增殖规律；结果发现:病毒在接种后12 h开始大量增殖，在18-36 h增殖最为迅速，在36-48 h病毒增殖达到最高峰；研究还发现在18 h 时Marc-145细胞开始释放病毒，48 h达最高峰。该结果为研究猪繁殖与呼吸综合征病毒的致病机理及利用细胞毒生产疫苗提供试验基础。

关键词: 氮肥, 氮肥, 钾肥, 花椰菜, 贮藏, 品质因子, 养分含量

Abstract:

Abstract: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) could grow in PAM, the cell line of CL2621 and Marc-145, but CPE is not distinctness, and the replication disciplinarian is still clearly unknown. This experiment investigated that the replication disciplinarian of PRRSV in the line of Marc-145 using Fluorescence Quantitative PCR. The results found that the virus begin to replicate in a great speed at the 12 h after inoculation and its growth is fastest between the 18-36 h, reaches peak at 48 h. These experiments supplied the production of vaccine and helps to do research on viral pathology of PRRSV.