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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 83-85.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

丙烯酰胺人工抗原的合成及其多克隆抗体的制备

张红星,高美琴,张馨如,刘慧   

  • 收稿日期:2009-06-15 修回日期:2009-06-17 出版日期:2009-08-20 发布日期:2009-08-20

Synthesis of Acrylamide Artificial Antigen and Preparation of Anti-Acrylamide Polyclonal Antibody

, , ,刘慧 LIU Hui   

  • Received:2009-06-15 Revised:2009-06-17 Online:2009-08-20 Published:2009-08-20

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摘要： 摘 要：利用偶联方法合成丙烯酰胺人工抗原，通过免疫方法获得抗丙烯酰胺多克隆抗体；应用戊二醛法将丙烯酰胺与牛血清白蛋白(BSA)进行偶联，并对这两种物质及其复合物进行紫外扫描，并用此复合物免疫兔子，利用间接酶联免疫吸附法测定抗体的效价；应用牛血清白蛋白与丙烯酰胺偶联人工抗体成功，抗体效价大于8100；应用人工抗原免疫成功制备抗丙烯酰胺多克隆抗体。

关键词: 稻瘟病菌, 稻瘟病菌, Pot2, rep-PCR, 有性后代随机群体

Abstract: Abstract: Artificial antigen of acrylamide was synthetized by couple method, and anti- acrylamide polyclonal antibody were obtained by immunizing method. The Glutaraldehyde method was used to conjugate Acrylamide to Bovine Serum albumin(BSA), and artificial antigen, acrylamide and BSA were respectively taken on UV spectrum scan. Rabbits had been immunized with artificial antigen, and the titer antiserum from those rabbits were testified by indirect enzyme-linked immunosorbent assay; The result showed that artificial antigen was synthesized successfully between Acrylamide and BSA, and titer of anti-acrylamide serum was above 8100; Anti-Acrylamide polyclonal antibody was prepared by immunizing method of artificial antigen.