关键词: 模拟光条件, 模拟光条件, 四川桤木, 品系, 光合特性

Abstract: The expression vector pFYC-AtNHX1containing AtNHX1 and pCAMBIA3300 containing bar gene mixed in equal concentrations were co-transformed into tomato varieties via pollen-tube pathway. 12 AtNHX1 positive plants were selected by spraying 300mg L-1 Basta on transgenic tomato leaves and by PCR assay of specific AtNHX1 for PPT resistant plants. The presence and integration of the AtNHX1 in transgenic tomato genome were confirmed by Southern blotting. Expression of the AtNHX1 in transgenic positive plants was determined by RT-PCR .The salt tolerance of transgenic plants were enhanced when exposed to 150 mmol?L-1 NaCl. The results demonstrated that AtNHX1 gene could improve the salt tolerance of tomato effectively. In order to investigate whether the AtNHX1 gene transformation can enhance the NaHCO3 tolerance of the T1 transgenic plants, relative conductivity in leaves of the transgenic plant and control were estimated when they were cultured under the 80mmol/L NaHCO3 (pH 8.25)stress. The results indicated that the intergration of AtNHX1 gene enhanced the resistance to the NaHCO3 stress of the transgenic tomato. Breeding salt and alkali tolerant transgenic AtNHX1 tomato cultivars is one approach to reducing the deleterious effects of saline environment on tomato.