关键词: 云南省, 云南省, 设施园艺, 发展, 对策

Abstract:

In this study, the preferential RAPD technology for walnut genetic analysis was successfully developed with utilization of 11 nt length primers instead of 10 nt ones and accurate selection of annealing temperatures, which was the results from the study on 30 walnut cultivars. Based on RAPD fingerprint quality, 18 11nt-primers have been screened from 40 ones for further walnut genetic analysis. These 18 primers could amplify 111 PCR bands ranging from 500 to 3050 bp in size, among which 77 were polymorphic bands and the polymorphism was 69.4%. The genetic relationships among the 30 walnut cultivars were analyzed using these RAPD polymorphic markers and the phylogenetic tree of these cultivars was also constructed, in which 10 distinct groups were clustered.