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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (5): 254-256.

所属专题： 食用菌； 食用菌

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

虎奶菇菌丝体自溶条件的优化研究

黄碧芳,陈婷,黄杰,邱莉莉,林文雄   

  • 收稿日期:2009-10-14 修回日期:2009-12-09 出版日期:2010-03-05 发布日期:2010-03-05
  • 基金资助:

    虎奶菇菌丝自溶的差异蛋白组学分析

Study on the optimization of the autolytic conditions of Pleurotus tuber-regium (Fr.) Sing mycelium

  • Received:2009-10-14 Revised:2009-12-09 Online:2010-03-05 Published:2010-03-05

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摘要：

自溶现象广泛存在于各种微生物中，但有关食药用菌虎奶菇（Pleurotus tuber-regium (Fr.) Sing.）菌丝体自溶的研究国内外均未见报道。本文利用液体发酵的方法来培养虎奶菇菌丝体，并分别研究了不同温度、pH值与渗透压、O2供应等因素对其菌丝体自溶的影响。结果表明，在本实验条件下，虎奶菇菌丝体较佳的单因子自溶条件分别为温度40℃、pH 9.0、NaCl浓度3-4%及无氧条件。本实验结果为虎奶菇菌丝体自溶机制的深入研究奠定了基础。

关键词: 烟草, 烟草, 控烟, 公共政策, 价值取向, 烟草

Abstract:

The phenomenon of autolysis is widespread among various kinds of microbes. However, there is hitherto no report on the autolysis of Pleurotus tuber-regium (Fr.) Sing. Liquid fermentation method was applied to culture the Pleurotus tuber-regium (Fr.) Sing mycelia and the effects of temperature, pH, osmotic pressure and oxygen supply on the autolysis of Pleurotus tuber-regium (Fr.) Sing mycelium were examined. The results indicated that the optimal conditions for the mycelium autolysis were 40℃, pH 9.0, 3-4%NaCl and anaerobic fermentation. This study provides the basis on which the further investigation of the Pleurotus tuber-regium (Fr.) Sing mycelium autolysis mechanism can be conducted.