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中国农学通报 ›› 2010, Vol. 26 ›› Issue (5): 93-96.

所属专题: 小麦

• 农学 农业基础科学 • 上一篇    下一篇

黑粒小麦的选育及其抗氧化特性

张东阳,李集临,张杰   

  • 收稿日期:2009-10-14 修回日期:2009-11-23 出版日期:2010-03-05 发布日期:2010-03-05

  • Received:2009-10-14 Revised:2009-11-23 Online:2010-03-05 Published:2010-03-05

摘要:

摘要:本文选育的黑粒小麦为黑皮黑胚乳,含有丰富的水溶性黑色素的小麦,国内外对黑色素作了一定的开发研究,但黒粒小麦清除二苯基-2-苦肼基自由基的研究还很少。本研究以普通小麦和抗坏血酸为对照,通过DPPH 法测定黑粒小麦色素粗提物体外抗氧化、清除自由基能力。研究结果表明:黑粒小麦黑色素粗提物具有呈量效关系的抗氧化活性,其清除自由基能力远大于普通小麦,但小于抗坏血酸。普通小麦、黑粒小麦3-2、3-3、3-4、3-11、3-13、3-33、3-36、3-37、3-39、10-38和Vc清除50% DPPH 自由基的有效浓度(EC50)分别为689.05、39.89、71.51、58.57、48.13、33.99、105.27、62.15、30.97、35.15、35.09、0.196 mg/ml,其自由基清除能力AE 分别为0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2。这些为进一步研究、开发和利用黒粒小麦黑色素奠定理论基础。

关键词: 烤烟, 烤烟, 烘烤, 密集烤房

Abstract:

Abstract: Our Black - grained Wheat contains black endosperm and rich water-soluble pigment, which has been studied both at home and abroad.There is almost no study on free radical scavenging effect of these pigments in Black - grained Wheat by DPPH. Using Chinese Spring and Vc as the control, We evaluate free radical scavenging and anti-aging effect of pigment crude extract in Black- -grained Wheat by DPPH. The result shows that pigment in Black - grained Wheat has dose-dependent effect on free radical scavenging, The ability of free radical scavenging and anti-aging of the pigment crude extract from Black - grained Wheat was greater than those of Chinese Spring, but lower than those of Vc. EC50 ( 50 % the initial DPPH ? concentration) of Chinese Spring、Black- -grained Wheat3-2、3-3、3-4、3-11、3-13、3-33、3-36、3-37、3-39、10-38 and Vc was 689.05、39.89、71.51、58.57、48.13、33.99、105.27、62.15、30.97、35.15、35.09、0.196 mg/ml DPPH ?respectively , and the antiradical efficiency (AE) was 0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2.All of this lay a foundation for further study, exploit and utilize pigment in Black - grained Wheat.