关键词: 辣椒, 辣椒, WRKY, 转录因子, 生物信息学

Abstract:

【Objective】The effect of a traditional chinese medicine preparation（TCMP）on the change of nonspecific immunity,such as mortality,relative percent survival, spleen index and antioxidase in Monopterus albus was evaluated in this study. 【Methods】All synbranchoid eels were divided into six groups,and solution was injected into abdominal cavity of each eel at the 3th day and the 9th day.The first group, acting as negative-blank contral group,was just injected intraperitoneally with normal saline.The second group was injected with cyclophosphamide. One medication group was injected with cyclophosphamide and astragalus injection at the same time as drug-treated control fish.The other three groups were injected with cyclophosphamide and plus Yupingfeng power simultaneously.Thirty-three eels were treated with 0.8ul/g?BW plus Yupingfeng power ,which was made up of Huangqi,Baizhu,Fangfeng,Dangshen and Gancao,as low-dosage-drug-treated fish, middle-dosage-drug-treated fish were injected with 1.6ul/g?BW plus Yupingfeng power and the high-dosage-drug-treated fish was injected with 3.2ul/g?BW plus Yupingfeng power. Counting of mortality rate and spleen index,and detecting the change of antioxidant enzyme activities at the 15th day of experiment. 【Results】The results indicated that,compared with the masculine-blank contral group, the mortality rates of each treated group were decreased,the level of spleen index was elevated,the activities of SOD,CAT and GSH-Px increased obviously,and the low-dosage-drug-treated group showed the best effect.Overall,these results as mentioned above demonstrated that the Plus Yupingfeng Power can enhance the level of spleen index,and also bring the activities of SOD,CAT and GSH-Px going up significantly. 【Conclusion】The preparation has a effective immunopotentiator against the experimental immunosuppression of Monopterus albus.