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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 250-254.

所属专题： 生物技术； 水稻

• 植物保护 农药 • 上一篇    下一篇

用SSR标记法对黑龙江省稻瘟菌无毒基因的检测

张崎峰1,靳学慧2,蔡鑫鑫2,巩双印1,刘显元1   

  • 收稿日期:2009-12-03 修回日期:2010-01-06 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    黑龙江省科技攻关项目;黑龙江省农垦总局科技攻关项目

Identification of Magnaporthe oryzae avirulence gene in Hei Longjiang Province with SSR Marker

Zhang Qifeng1, Jin Xuehui2, Cai Xinxin2, Gong Shuangyin1, Liu Xianyuan1   

  • Received:2009-12-03 Revised:2010-01-06 Online:2010-05-20 Published:2010-05-20

PDF (PC)

881

摘要：

使用与无毒基因Avr-pit、Avr-pia、PRE1紧密连锁的3个SSR标记对黑龙江省不同地区的195个稻瘟菌菌株的DNA进行PCR扩增。结果表明，各无毒基因在黑龙江省的出现频率有明显差异，无毒基因Avr-pit的出现频率为43.59%，无毒基因Avr-pia出现频率36.92%，无毒基因PRE1的出现频率为30.26%。黑龙江省稻瘟病菌的群体结构复杂，在不同地区甚至相同地区的菌株中无毒基因的存在情况都有明显的差异。

关键词: 农业面源污染, 农业面源污染, 生态补偿, 措施和政策

Abstract:

Using three SSR marker which was closely linked to the avirulence gene Avr-pit，Avr-pia and PRE1 for PCR amplification of DNA in 209 Magnaporthe oryzae strains of different region Heilongjiang province. The result showed that the frequency of different avirulence gene is significant different in Heilongjiang province, and the appearance frequency of these three avirulence gene Avr-pit, Avr-pia and PRE1 were 43.59%,36.92% and 30.26%. The group structure of Magnaporthe oryzae was complex in Heilongjiang Province．The distribution of avirulence gene varied in different regions even in the same region．